A Novel Approach to Increase Omega-3 Polyunsaturated Fatty Acids for Cardiometabolic Disease Management

Activity: Talk or presentation types › Oral presentation

Description

The health benefits of omega-3 polyunsaturated fatty acids (PUFAs) are widely recognized in cardiometabolic disease management. Replacement of dietary saturated fatty acids (FA) with PUFAs reduces risks of cardiovascular diseases and type 2 diabetes. The aim of this study was to investigate a novel approach to increase PUFA levels for cardiometabolic disease management and measure PUFA and respective acylcarnitine (PUFAC) concentrations in experimental models of obesity using the LC-MS/MS method.. Plasma samples were collected from low-density lipoprotein receptor knock-out mice fed for 16 weeks of PUFA-rich high-fat diet (HFD) and standard HFD-treated control mice. Additional experiments to determine plasma PUFA concentrations were performed in Zucker rats, which were given daily oral doses of water or 10 mg/kg acylcarnitine level-lowering compound methyl-GBB for 12 weeks. A simple protein precipitation extraction (PPE) with ACN/MeOH (3:1, v/v) was used to extract plasma samples and the resulting extracts were analyzed using reversed-phase UPLC–MS/MS. The mobile phase consisted of gradient elution of 0.1% formic acid in water and ACN. Separation was achieved on an Acquity UPLC BEH C18 column. . PUFA-rich diet prevented the development of atherosclerosis in mice and increased DHA and EPA concentrations 5 and 38 times, respectively, while corresponding PUFAC levels increased 12 and 23 times, respectively. Methyl-GBB treatment inhibited fatty acid oxidation and increased EPA and DHA concentrations 2- fold in the Zucker fa/fa rat plasma compared to control groups. . In conclusion, PUFA intake induces a similar increase in blood plasma concentrations of PUFAs and respective acylcarnitines. The developed analytical method could be used in clinical studies to validate PUFA and PUFAC plasma levels as markers of PUFA intake. The combination of omega-3 PUFA intake and inhibition of their metabolism is a very promising approach to increase omega-3 PUFA content and reach cardioprotective levels.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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