Bacterial Colonisation and Role of Bacterial Biofilms in the Upper Respiratory Tract

Activity: Talk or presentation types › Oral presentation

Description

To evaluate the tonsillar crypts as a potential reservoir for clinically important pathogens and analyse biofilm formation and antibacterial susceptibility of isolated strains. . In a prospective cohort study tissue samples were obtained from tonsillar crypts of 91 healthy students. Samples were acquired with a brush and cultivated on Columbia blood, Mannitol salt, MacConkey, Sabouraud dextrose, Brucella blood and Chocolate agar. Bacteria were identified with MALDI–TOF mass spectrometer. Antimicrobial susceptibility was detected using disk diffusion test. Crystal violet assay was used for assessment of biofilm production.. Oropharyngeal microbiota were cultivated from 37 participant samples (40.7%). The S. aureus predominated, it was detected in 41 (45%) participant samples. K. pneumoniae was isolated in 7 (7.7%) samples, Acinetobacter spp. were isolated in 5 (5.5%) samples, and P. aeruginosa was isolated in 2 (2.2%) samples. S. aureus strains were predominantly biofilm producers: 25 out of 41 (61%) S. aureus strains were moderate or strong biofilm producers, and 14 out of 41 (34.1%) S. aureus strains were weak biofilm producers, but 2 out of 41 (4.9%) S. aureus strains were biofilm nonproducers. Among the Gram-negative bacteria, 6 out of 15 (40%) strains were moderate or strong biofilm producers and 6 out of 15 (40%) strains were weak biofilm producers, but 3 out of 15 (20%) strains were biofilm nonproducers. The tested S. aureus, K. pneumoniae, P. aeruginosa, and Acinetobacter spp. strains were sensitive to commonly used antibiotics (cefoxitin, amoxicillin – clavulanic acid, clindamycin, or ciprofloxacin). One S. aureus strain was MRSA.. Biofilms seem to be a naturally existing form of pathogenic bacteria that are colonizing human tissues of healthy individuals. S. aureus, K. pneumoniae, P. aeruginosa, and Acinetobacter spp. can be found in the tonsillar crypts of healthy individuals, and it is therefore most likely that opportunistic tonsillar infections originate from the tonsillar crypt microbiota.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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