Biofilm Formation on Different Smooth and Textured Silicone Breast Implant Biomaterials

Activity: Talk or presentation types › Oral presentation

Description

The aim of this research is to investigate the ability of Staphylococcus epidermidis ATCC 12228, Pseudomona aeruginosa ATCC 27853 and Candida albicans ATCC 10231 to form biofilms – on smooth, micro-textured and macro-textured breast silicone biomaterial in an in vitro study.. A total of 90 samples of silicone biomaterial outer shells were used, with (n = 30) for each type of biomaterial – smooth, micro- and macro-textured. In each of the biomaterial groups, half of the samples (n = 15) were used to determine the biofilm formation after 2-hour incubation in microorganism suspension and the other half to determine biofilm formation after 24-hour incubation in microorganism suspension on the active surface of the samples. Later the samples were sonicated for one minute in 44 kHz frequency, then the suspense was mixed and cultured on Trypticase soy and Sabouraud agar for 48 hours.. The most intense biofilm formation after 2 H was shown by P.aeruginosa ( smooth 454 CFU/ cm2, micro 1135 CFU/ cm2, macro 2383 CFU/ cm2 on average), followed by S.epidermidis (454 CFU/ cm2, 340 CFU/ cm2, 794 CFU/ cm2 on average respectively) and C.albicans (340 CFU/ cm2, 113 CFU/ cm2, 227 CFU/ cm2 on average respectively). Increased biofilm formation after 24 H was demonstrated by P.aeruginosa ( smooth 1x106 CFU/ cm2, micro 3x106 CFU/ cm2, macro 1,1x 107 CFU/ cm2 on average), followed by S.epidermidis (4,5x105 CFU/ cm2, 1,3x106 CFU/ cm2, 3,6x106 CFU/ cm2 on average respectively) and C.albicans (1x104 CFU/ cm2, 1,6x104 CFU/ cm2, 3,6x104 CFU/ cm2 on average respectively).. The strongest biofilm formation after 2 H was to the surface of macrotextured implant. Among microorganisms, which showed the highest biofilm formation rates after 24 H were P.aeruginosa. The most intense biofilm formation was on the macro-textured implant, then on the micro-textured and then on the smooth.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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