Characteristics of Enterococcus Resistance Rate and Presence of Van Genes in Resistant Cultures from Two Multidisciplinary Hospitals in Latvia

Activity: Talk or presentation types › Oral presentation

Description

A common pathogen causing various infections is Enterococcus. It can cause various infections if migrating to other than gastrointestinal tract locations throughout a human body. In majority of cases such infections are uncomplicated to manage, but problems arise if enterococci are becoming resistant against first line treatment. In case of infections caused by a very resistant enterococcus, antibacterial agent vancomycin is administered. There is rising concerns about an increasing rate of resistance against vancomycin. Van genes are responsible for enterococci resistance against vancomycin. There are 9 clusters of van genes discovered in decent decades- vanA, vanB, vanC, vanD, vanE, vanG, vanL, vanM, and vanN. The most common van gene in Europe ir vanA, followed by vanB and VanC gene. The aim of our study was to investigate Enterococcus samples, the rate of resistance against vancomycin and particular van genes presented in the case of such resistance.. Enterococci containing cultures from two multidisciplinary hospitals in Latvia were analysed. Full genome sequencing was performed with Illumina MiSeq, with a protocol of Illumina DNA Prep library. ResFinder data base was used to identify the resistance genes against vancomycin and other antibacterial agents. MS Excel program applied for performance of descriptive analysis. . 120 samples were analysed. 86 samples contained E. faecalis, 49-E. faecium, E. durans- 1, E. gallinarium- 1, and E. raffinosus-1. 86 samples were sensitive, but 34 samples were resistant against vancomycin. Van genes A, B, C, and M were detected. VanB- in 25 samples (E. faecium-18; E. faecalis-7), vanA-7 samples (E. faecium-6; E. raffinosum-1), vanC-1 (E. gallinarium), and vanM in one sample (E. faecium). E. faecalis resistance rate was 10.29%, E. Faecium- 50%. . The most common resistance gene found in samples was vanB gene. The highest resistance rate against vancomycin was detected in E. faecium.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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