Chemical Profiling of Phenolic Compounds from Sea Buckthorn Twigs

  • Līga Lauberte (Speaker)
  • Anna Andersone (Co-author)
  • Sarmīte Janceva (Co-author)

Activity: Talk or presentation types › Oral presentation

Description

Sea buckthorn (Hippophae rhamnoides L.) represent a rich source of natural compounds which are responsible for many multifunctional biological effects. In this work, we report the phytochemical composition of aqueous ethanol extracts of unexplored so far new promising plant material sea buckthorn twigs. We gather and systematize available information on identified phenolic with an emphasis on their potential biological activities.. Extracts were isolated by the convective extraction of twigs. The UHPLC-UV-TOF/MS was used for identification of individual polar monomeric and oligomeric compounds without modification. The total phenolic content was determined quantitatively using the Folin Ciocalteu reagent, with Gallic acid as the standard.. The extraction results showed that the yield of hydrophilic extractives from sea buckthorn samples is in the range of 15 to 30%. The profiling of secondary metabolites in the extracts of sea buckthorn twigs growing in Latvia by UHPLC-UV-TOF/MS showed that the chemical composition is rich with the polyphenolic compounds, mainly, proanthocyanidins. Total amount of polyphenols in extracts varies from 30 up to 50 GAE ∙ g on 100g dry extract depending on species. Amino acid derivatives such as serotonin, leucine and alanine derivatives, have been identified in the extracts. Flavonoids – catechin, quercetrin, their glycosides, proanthocyanidins from dimer to tetramer, organic acids (quinic acid, gallic acid, caffeic acid) were identified as well.. The results indicate that crude extracts were characterized by a good amount of phenolic compounds. These chemical compounds identified are recognized as biologically active compounds, namely, as antioxidants or antibacterial agents. This research was funded by ERDF project nr. Nr.1.1.1.1/19/A/146 “Biorefinery processing of sea buckthorn non-fruit biomass using innovative techniques and comprehensive analytical investigation, for obtaining prospective for Latvian bioeconomy high value-added products, including serotonin”.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

Related content