Development of Original Styrylpyridinium Derivatives as Fluorescent Dyes for Biomedicinal Applications

  • Plotniece, A. (Speaker)
  • Putrālis, R. (Co-author)
  • Ksenija Korotkaja (Co-author)
  • Laura Krasnova (Co-author)
  • Martins Rucins (Co-author)
  • Ilona Domracheva (Co-author)
  • Martins Kaukulis (Co-author)
  • Plotniece, M. (Co-author)
  • Arkadij Sobolev (Co-author)
  • Karlis Pajuste (Co-author)
  • Anna Zajakina (Co-author)

Activity: Talk or presentation types › Poster presentation

Description

Styrylpyridinium salts are used as fluorescent probes for biochemical, biophysical, molecular biology studies.[Dubur et al 1984; Xu et al, 2015] Styrylpyridinium compounds also possess antimicrobial properties.[Vaitkiene et al, 2020] The development of a new class of dyes with desirable photophysical properties is a challenge for the researchers working in this field.The aim of the study was synthesis and evaluation of physical, self-assembling and biological properties of a set of styrylpyridinium derivatives.. Styrylpyridinium derivatives were synthesised from the appropriate aldehydes and 4-picolinium salts according to Vaitkiene et al.[Vaitkiene et al, 2020] Dynamic light scattering (DLS) method was used for estimation of self-assembling properties of samples prepared by tin film hydration method.[Pajuste et al, 2013] Cytotoxicity of compounds in vitro was assessed by the MTT test on two tumor cell lines – HT-1080 and MH-22A and normal mouse fibroblasts 3T3.[Strokes et al, 2008]. Fluorescent microscopy was used to analyze cell fluorescence.. Set of original styrylpyridinium dyes with variation of aldehyde and N-alkyl moieties was obtained. Several compounds from the set of obtained dyes formed nanoparticles with the average size around 120 nm of freshly prepared 1mM aqueous solutions. The compounds show an intensive fluorescence, especially in lipophylic medium. Some from the tested compounds have rather high cytotoxicity to tumor cell lines HT-1080 and MH-22A; at the same time basal cytotoxicity to 3T3 cell line was in a range from toxic to harmful. Fluorescent microscopy of cells preincubated with some compounds revealed an intense red fluorescence monitored within at least 5 days of cultivation. Structure-activity relationships will be discussed.. Variations of structure and alkyl moieties at synthesized compounds strongly affect the properties of the tested styrylpyridinium derivatives. Funded by the Latvian Council of Science project lzp-2021/1-0283.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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