Diagnostic Accuracy of Multiple Biomarkers in Predicting The Severity of Acute Appendicitis in The Emergency Department

Activity: Talk or presentation typesOral presentation


Aim of the study:
Since acute appendicitis (AA) in children can be treated differently according to the severity of the disease, we investigated whether the combined diagnostic model of interleukin-6 (IL-6), leucine-rich alpha-2 glycoprotein 1 (LRG1), neutrophil gelatinase-associated lipocal (NGAL) could distinguish between acute uncomplicated appendicitis (AuA) and acute complicated appendicitis (AcA) and a control group (Ctr).

Materials and Methods:
In this prospective single-centered cohort study, IL-6, LRG1, NGAL in serum were assayed preoperatively. Children aged seven to 18 years old were divided into three groups: AcA, AuA, and Ctr. The Ctr included patients without any suspected inflammatory processes in the respiratory, renal or gastrointestinal tract,. The predictive values of biomarkers were evaluated by receiver operating characteristics curve (ROC) and binary logistic regression models. Two different models were analyzed – AA vs Ctr and AcA vs AuA.

A total of 153 participants were enrolled, including AcA (n = 52), AuA (n = 45) and Ctr (n = 56). The combined diagnostic model of IL-6, LRG1, NGAL in serum was established by binary logistic regression analysis. The ROC curve showed that combined diagnostic model AA vs Ctr reached a sensitivity of 89.3 %, a specificity of 92.8 % and an area under the curve of 0.96 (95% CI 0.93-0.99, p
The combined diagnostic model of IL-6, LRG1, NGAL at the emergency department (ED) may provide a new approach for the differentiating between AcA and AuA, and for the diagnoses of AA as opposed to other causes of abdominal pain.
Period29 Jun 2022
Event title23rd Annual Congress of the European Paediatric Surgeons’ Association (EUPSA)
Event typeCongress
Conference number23
OrganiserEuropean Paediatric Surgeons' Association
LocationTelaviv, IsraelShow on map
Degree of RecognitionInternational


  • pediatric apendicitis
  • diagnostics