Diagnostic Accuracy of Potassium Hydroxide Smear Relative to Fungal Culture – Single Center Experience

  • Kristiana Rocane (Speaker)
  • Vera Bondareva (Co-author)
  • Linda Maule (Co-author)
  • Storoženko, J. (Co-author)

Activity: Talk or presentation types › Poster presentation

Description

Rapid detection of dermatophytosis is key for timely initiation of treatment. Potassium hydroxide smear (KOH) remains an integral test for the rapid detection of dermatophytosis even with the prospect of modern mass spectrometry methods. The review of diagnostic accuracy of traditional methods is needed for an algorithmic approach to laboratory detection of dermatophytosis.. An observational cross-sectional study design was selected. The study enrols 5568 cases of fungal diagnostic testing for suspected dermatophytosis. All fungal testing reports included both KOH smear microscopy and fungal culture and were performed in Riga East University hospital from October 2019 to October 2022. Fungal culture was selected as the reference standard method for the diagnostic accuracy comparisons.. KOH smear microscopy was negative in 74.1% of the cases. Fungal culture was negative in 80.5% of the cases. Cultures found Trichophyton spp. in 17.7% of cases and Microsporum spp. in 1.8% of the cases. Trichophyton rubrum and Microsporum canis and Trichophyton mentagrophytes were the most common among positive fungal cultures, found in 82.5%, 6.9% and 4.7% respectively. KOH smear of Trichophyton spp. had a sensitivity, specificity, and accuracy of 87.1%, 88.1%, and 88.0%, respectively. KOH smear of Microsporum spp. had a sensitivity, specificity, and accuracy of 55.6%, 88.1%, and 87.5%, respectively. KOH smear microscopy in Trichophyton spp. cases had an AUC of 0.872 (CI95 0.859-0.886, p
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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