Description
Background: Life-threatening sepsis resulting from an uncontrolled inflammatory response is the leading cause of mortality in the ICU. Despite advances in treatment, predicting outcomes remains difficult. Personalized care is desirable to increase the chances of survival. Various factors such as patient characteristics, metabolomic profile, oxidative stress marker, and platelet count have been investigated to predict adult sepsis mortality. Still, the accuracy of clinical prognosis is limited. We aim to investigate differences in cell-free blood DNA (cfDNA) quantities, including bacterial DNA, in survivors of refractory septic shock (SS) and non-survivors (NSS).Methods: The study enrolled 33 survivors (age 63 ± 15) and 29 non-survivors (age 66 ± 14) of septic refractory shock, and clinical data was collected upon admission. CfDNA from whole blood plasma was isolated with QIAamp Circulating Nucleic Acid Kit (Qiagen), dsDNA and ssDNA concentrations were measured with Qubit respective kits. Bacterial DNA was detected with broad-range 16S rDNA qPCR and relative concentrations were calculated from the standard curve.
Results: We have found only mild differences in dsDNA (higher in NSS) and ssDNA (higher in SS) values between the groups (p=0.08 and p=0.38, respectively). As for 16S rDNA, NSS group had significantly higher bacterial DNA concentrations (0.0214 ± 0.0496 ng/ml for SS and 0.1907 ± 0.4308 ng/ml for NSS) (p<0.05). Gender has a significant effect - females in NSS group have a higher bacterial DNA load (0.3606 ± 0.5782 ng/ml) than both males in NSS (0.0320 ± 0.0695 ng/ml) and females in SS groups (0.0377 ± 0.0843 ng/ml) (p<0.05 and p=0.0679, respectively). No correlation was found between bacterial DNA load and ssDNA or dsDNA concentrations in cfDNA from blood.
Conclusion: We show higher bacterial DNA concentrations in cfDNA from blood in non-survivor females, which can be considered in predicting the possible refractory septic shock outcome.
Period | 4 Mar 2024 → 7 Mar 2024 |
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Event title | 3th International Symposium on Circulating Nucleic Acids in Plasma and Serum |
Event type | Symposium |
Conference number | 31 |
Organiser | Vienna Medical Academy |
Location | Graz, AustriaShow on map |
Degree of Recognition | International |
Keywords
- cfDNA
- refractory septic shock
- metagenome