Evaluation of Antioxidant and Anti-Inflammatory Activity of Oat Milk and Yogurt Extracts

  • Sīle, I. (Speaker)
  • Razživina, V. (Co-author)
  • Melita Ozola (Co-author)
  • Karina Juhnevica Radenkova (Co-author)
  • Vitalijs Radenkovs (Co-author)
  • Solveiga Grinberga (Co-author)
  • Edgars Liepiņš (Co-author)
  • Dambrova, M. (Co-author)

Activity: Talk or presentation types › Oral presentation

Description

In recent years, plant-based milk and yogurt alternatives, such as oat milk, have become highly popular. Oats are a great source of antioxidant plant compounds, such as ferulic acid (FA) and isoferulic acid (IFA). The aim of the study was to determine and compare the biological activity of oat milk and oat yoghurt extracts. . The determination of the antiradical activity of oat milk extract, oat yogurt extract, FA and IFA standard substances was carried out using DPPH method. The effects of the samples on cell proliferation and viability were tested using the MTT assay. The antioxidant activity of the studied samples was evaluated in cell cultures using the dichloro-dihydrofluorescein method. The expression of CD80 and CD86 in bone marrow-derived macrophages (BMDM) was examined by flow cytometry analysis. Lipopolysaccharide (LPS) was used to induce pro-inflammatory signaling.. The most pronounced antiradical activity was exerted by FA (EC50 = 23.6 ± 4.1 µM) and oat milk extract (EC50 = 24.8 ± 6.3% of the solution). Based on the results of the MTT assay, after incubation with oat milk and oat yogurt extracts, the samples significantly promoted macrophage viability (BMDM) but decreased CaCo-2 cell viability by ~20%. FA and IFA did not reduce the viability of CaCo-2 cells and BMDM at concentrations up to 250 μM. Oat yogurt extract, FA, and IFA reduced ROS production in BMDM by 46%, 26%, and 22%, respectively. In CaCo-2 cells, FA and IFA at 100 µM concentration significantly reduced ROS by 61% and 60%, respectively. Pre-incubation of BMDM with oat milk extract reduced macrophage population M1 (pro-inflammatory) by 50%, FA and IFA at 100 µM concentration reduced the M1 population by 35% and 12%, respectively, compared to the LPS control.. The analyzed oat milk extract showed significantly higher biological activity in vitro compared to the oat yogurt extract.
Period30 Mar 2023
Event titleRSU Research Week 2023: Research Week 2023 Rīga Stradiņš University
Event typeConference
OrganiserRiga Stradins University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational