Evaluation of Various Tissue Factors in the Bone of First and Second Time Performed Surgery (Osteoplasty and Rhinoplasty) in Cleft Lip Palate Patients

Activity: Talk or presentation types › Oral presentation

Description

Cleft lip and palate (CLP) deformities are among the most prevalent craniofacial birth abnormalities. The purpose of our research was to ascertain how tissue factors appeared and varied in the bone tissue of CLP patients after primary and secondary bone surgery.. Maxillary bone was obtained from CLP patients during osteoplasty or rhinoplasty. The primary operated CLP patients group consisted of 14 patients, and the secondary operated CLP patients group consisted of 22 patients. Immunohistochemistry was performed with: OPN, OPG, OC, IL-1α, IL-10, HBD-2, HBD-3, BMP2/4, bFGF, TGF-β1, MMP-2, MMP-8, MMP-9, TIMP-2, Runx2, Wnt3a and the TUNEL method. The semi-quantitative census method was used for the quantification of structures. The Spearman rank correlation coefficient and Mann- Whitney U test were used for the statistical analysis.. The HBD-2, HBD-3, IL-1α, OC, MMP-2, MMP-9, BMP 2/4, TGF-β and TUNEL demonstrated higher numbers in second time operated patients. There was no difference between the median numbers of IL-10, OPG, OPN, MMP-8, TIMP-2, bFGF, Runx2 and Wnt3a. A significantly higher number of MMP-2 (U=95.500; p=0.05) positive osteocytes were observed in the second time operated CLP patient group, where MMP-2 also showed strong, positive correlations with HBD-2 (rs= 0.752; p
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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