Examination of Inflammatory and Anti-Inflammatory Cytokines and Factors in Milk of Dairy Cattle with Different Health Status of the Udder

  • Ksenija Šerstņova (Speaker)
  • Pilmane, M. (Co-author)
  • Melderis, I. (Co-author)
  • Lukasz Gontar (Co-author)
  • Maksymilian Kochanski (Co-author)
  • Andzelika Drutowska (Co-author)
  • Beatriz Prieto Simón (Co-author)

Activity: Talk or presentation types › Oral presentation

Description

The aim of this study was to detect various inflammatory and anti-inflammatory cytokines and factors in cattle milk from an agricultural farm and examine the change in the levels of these possible biomarkers between healthy cows and animals with subclinical and clinical mastitis.. Based on the health status of each animal in a purebred Polish Holstein Friesian cows herd, fifteen heads of dairy cattle were selected and divided into three groups: five cows were clinically healthy with low SCC in udder quarter milk; five animals with medium SCC were categorized as having subclinical mastitis, and the last five with high SCC had symptoms of clinical mastitis. The following analyses of milk samples were performed: the somatic cell count (LactoScan SCC), bacteriological examination (ISO 4833:2013 standard), and immunocytochemical analysis of inflammatory (IL-1α, IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-α, and IFN-γ) and anti-inflammatory (IL-2, IL-10, β-defensin-2, β-defensin-3, TGF-β1, and Cathelicidin LL37), and SPSS for statistical tests.. Both the mean SCC and total bacteria count increased in subclinical and clinical mastitis cases. Milk samples from all animal groups contained Streptococcus uberis and Staphylococcus aureus, as well as Streptococcus agalactiae, E. coli, and a total of Klebsiella, Enterobacter, and Citrobacter spp. Both the inflammatory and anti-inflammatory proteins in the milk of healthy cows showed the highest mean positive cell values; thus, IL-2, IL-4, IL-10, IL-12, IL-13, IL-17A, TNFa, IFNy, β-Def2, and β-Def3 showed a significant difference between healthy and subclinical and/or mastitis-affected animals. Immunoreactivity for inflammatory IL-12 and INF-γ and anti-inflammatory IL-10 and β-def3 was found to be useful in distinguishing mastitis cases from healthy animals. As a result, these molecules could possibly be used as biomarkers for the early detection of subclinical and clinical mastitis.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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