Findings of Different Tissue Factors and Apoptosis in Primary Obstructive Megaureter

Activity: Talk or presentation types › Oral presentation

Description

Primary obstructive megaureter (POM) morphogenesis is not fully known. Some studies showed that there are increased amounts of connective tissue, hypertrophy of outer muscle layer, and atrophy of inner muscle layer in ureteral wall. Very few studies have investigated the immunohistochemical profile of POM. In this study, we evaluate several factors that participate in the regulation of ureters growth and development.. The study material was obtained from 11 children aged from one month to 15 years. Three control samples were obtained from children not associated with megaureters from their distal part of the ureter. Apoptosis was detected by terminal dUTP nick-end labeling (TUNEL) reaction. Protein gene product 9.5 (PGP 9.5), nerve growth factor receptor (NGFR), transforming growth factor beta 1 (TGFβ1), fibroblast growth factor receptor 1 (FGFR1), matrix metalloproteinase 2 (MMP-2), angiotensin 2 receptor type 2 (AT2R2), and sonic hedgehog (SHH) protein were detected using immunohistochemistry methods and their relative distribution was evaluated by means of the semiquantitative counting method.. The megaureter material revealed transitional epithelium with scattered vacuolization, submucosa with inflammatory cells, chaotically organized and focally vacuolized muscle layers, and adventitia. Appearance of MMP-2, FGFR1, SHH, and apoptosis prevailed, but TGFβ1 positive cell number was lower in the patient group. Very strong positive correlations were observed between MMP-2 in epithelium and endothelium (rs = 0.867; p
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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