Interaction of Antibiotics and Bacteriophages in Bacterial Biofilms

Activity: Talk or presentation types › Oral presentation

Description

The increasing resistance to antibiotics has become one of the most important global health care problems. The ability of bacteria to form a biofilm is an important resistance mechanism. Studies indicate that bacteriophages can be used as an alternative to antibiotics or as combination with antibiotics with some limitations. The effect of combinated therapy can be synergistic, additive, or antagonistic, which is directly related to the mechanism of action of both antibacterial agents.. Evaluation of bacterial strains isolated from patients and reference strains were used. Lytic phages and antibiotics were used as antimicrobial agents. The antibacterial sensitivity was determined using disk diffusion test and broth microdilution test to detect minimum inhibitory concentration (MIC). Formation ability was determined by the Crystal violet assay. Bacterial susceptibility to phages was evaluated according to the spot test. The MIC and minimum biofilm eradication concentration (MBEC) of phages and antibiotics were determined with a Calgary device.. Phage and antibiotics can be used as separate therapies that can follow each other or combined together simultaneously. The sources of the literature and the results obtained in our study show that the order in which the therapy is used is essential: the best effect is observed if the phages are used first and antibiotics are applied only afterward. The interaction of antibiotics and phages should be assessed and may vary according to action mechanism of antibiotic and phage-bacterium interaction.. Bacteriophages and antibiotics can enhance each other's effects if the combination is used appropriately, but they can also act as antagonists. Such combinations can be used as alternatives in cases of multidrug-resistant bacteria, but each situation must be evaluated individually.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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