MicroRNAs as Potential Biomarkers in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

Activity: Talk or presentation types › Oral presentation

Description

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) continues to cause significant morbidity worldwide, and many individuals with ME/CFS symptoms remain undiagnosed due to the lack of diagnostic biomarkers. Recent evidence suggests a role of Herpesviruses (e.g. Human Herpesvirus 6) as potential causative agents and their infection has been widely reported to profoundly impact the expression of microRNAs (miRNAs), short sequences of non-coding RNA whose dysregulation has been associated with a variety of diseases including ME/CFS. In addition, the recent identification of circulating miRNAs has gained interest in the search for potential biomarkers for ME/CFS diagnosis. The objective of this study was to investigate the expression of specific miRNAs in plasma samples from ME/CFS patients.. The MagMax mirVana Total RNA isolation kit, based on magnetic-bead technology (ThermoFisher Scientific) was used to extract miRNAs from plasma of 40 patients with ME/CFS and 20 healthy donors. TaqMan advanced miRNA cDNA synthesis kit (ThermoFisher Scientific) was used to prepare the cDNA template. TaqMan Advanced miRNA assays (ThermoFisher Scientific) were used for individual analysis of target miRNAs. Data analysis was performed using Applied Biosystem real-time PCR Analysis Modules on Thermo Fisher Cloud.. The procedure allowed to obtain highly sensitive and specific quantitative analysis of mature miRNAs in plasma specimens. In particular, investigated miRNAs included: miR-448, miR-124, miR-551b, miR-127-3p, miR-142-5p, miR-143-3p, miR-140-5p, miR-150-5p, miR-361-5p, miR-186-5p and ath-miR-159a. . The results may open the way for further validation of circulating miRNAs as new potential biomarkers in ME/CFS and increase the knowledge of the complex pathways involved in the ME/CFS.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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