Morphological Characterisation of Paediatric and Adult Acquired Cholesteatoma

Activity: Talk or presentation types › Oral presentation

Description

The aim of this study was to compare remodelling factors MMP-2, MMP-9, TIMP-2, TIMP-4, proliferation marker Ki-67, inflammation factor NF-κβ, pro- and anti-inflammatory cytokines IL-1 and IL-10, defensins HβD-2, HβD-4, angiogenetic factor VEGF and  Sonic hedgehog gene protein in a cholesteatoma’s tissue in children and adults.. Tissues were obtained from 21 children and 19 adults during cholesteatoma surgery. Tissues were immunohistochemically stained for MMP-2; MMP-9; TIMP-2; TIMP-4; Ki-67; NF-κβ; IL-1; IL-10; HβD-2; HβD-4; VEGF, Shh. The slides were analysed by light microscopy using a semi-quantitative method. Non-parametric statistical analysis – Kruskal-Wallis’s test was used to detect statistical differences between groups.. MMP-2 mean values were a few to moderate (+/++), MMP-9 – occasional (0/+), TIMP-2 – a few (+), TIMP-4 – moderate to numerous (++/+++) factor positive cells in both groups. Ki-67 and NF-κβ mean number was occasional (0/+) and few to moderate (+/++) positive cells respectively. A few to moderate (+/++) IL-1, IL-10 and HβD-2 containing cells, but occasional (0/+) HβD-4 positive cells were observed in both groups. Mean values of VEGF containing cells were few to moderate (+/++), but Shh positive cells averaged a moderate (++) in children and adults. There were no statistically significant differences in all cell factors between both groups. Similar correlations were observed in both study groups between MMP-2 in matrix and perimatrix (r=0.626 adults, r=0.918 children); between MMP-9 in matrix and perimatrix (r=0.635 adults, r=0.682 children) and TIMP-2 in matrix and perimatrix (r=0.697 adults, r=0.708 children).. The increased MMP-2 and TIMP-4 suggest the presence of intensive remodelation in non-age depending cholesteatoma. Almost equal expression of inflammatory factors IL-1 and NF-κβ and anti-inflammatory ones like IL-10, HβD-2 indicate the correct balance between above mentioned processes in cholesteatoma with involvement of moderate tissue ischemia. Finally, detection of Shh proves the persistent gene involvement in cholesteatoma.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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