Mycoplasma Hominis and Ureaplasma spp. Selective Culture Broth Uninterpretable Test Result Analysis

Activity: Talk or presentation types › Poster presentation

Description

M.hominis and Ureaplasma spp. laboratory diagnosis is mainly based on selective culture broth diagnostic tests. Uninterpretable test results are often obtained when analysing different genitourinary tract samples.The aim of the study was to investigate and identify the cause of an uninterpretable test result in M.hominis and Ureaplasma spp. selective culture broth assays.. In October and November 2022 619 vaginal, cervical and urethral women swab samples were tested for M.hominis and Ureaplasma spp. using selective urea – arginine culture broth assay. At the same time these samples were tested for microbiological examination. Materials were inoculated onto various selective media - Columbia CNA, Macconkey un Sabouraud’s dextrose agar and the identification of grown microorganisms were made using Bruker MALDI Biotyper sirius one system.. In the examination of 619 samples 226 were positive for either M.hominis or Ureaplasma spp.; 361 were negative for both microorganisms and 32 were uninterpretable. Further microbiological examination of the testing material that gave uninterpretable results showed that 7/32 samples were positive for multiple microorganisms: 1/7 C.albicans and A.ursingii; 1/7 C.albicans and S.agalactiae; 1/7 C.albicans and G.vaginalis; 1/7 S.agalactiae and E.faecalis; 1/7 S.agalactiae and K.pnemoniae; 1/7 S.agalactiae and E.coli; 1/7A.baumannii and E.hormaechei. Single isolation and identification were obtained in 25/32 uninterpretable results, where 8/25 were species of yeast, 6/8 were C.albicans; 1/8 was C.glabrata and 1/8  was M.guilliermondii. Gram negative rods were isolated and identified in 15/25 uninterpretable results, in which 7/15 were K.pneumoniae, 4/15 were E.coli and 4/15 were P.mirabilis. In 2/25 cases were isolated and identified E.faecalis and E.faecium.. In the analysis we obtained 6.16 % uninterpretable test results. The reason for uninterpretable results could be material contamination with other bacteria (especially S.agalactiae and K.pneumoniae) and fungi (C.albicans) that can split urea or arginine and lead to active infection or coinfection with M.hominis and/or Ureaplasma spp..
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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