In humans, infection with an intestinal parasite can be asymptomatic, but some
cases present with diarrhea and other signs of gastroenteritis. Molecular approaches
are becoming increasingly available for detecting intestinal parasites, molecular
methods demonstrate excellent sensitivity and specificity with respect to
conventional methods such as microscopy.
In 2019 – 2021 891 samples were tested in NRL of Latvia to detect parasites DNA
by Allplex GI-Parasites Assay, Seegene from stool samples.
We compared B.hominis, D.fragilis, Cryptosporidium spp., C. cayetanensis, E.
histolytica, G. lamblia DNA positive samples with microscopy.
We obtained 308/891 (34.6%) positive parasites DNA. 141/308 (45.8%) B.hominis
DNA was found; D.fragilis DNA - 91/308 (29.5%); Cryptosporidium spp DNA - 9/308
(2.9%); G.lamblia DNA - 2/308 (0.7%); C. cayetanensis DNA - 1/308 (0.3%);
E.histolytica DNA - 0/308; B.hominis + D.fragilis DNA - 57/308 (18.5%); B.hominis +
Cryptosporidium spp. DNA - 3/308 (1%); Cryptosporidium spp. + D.fragilis DNA -
2/308 (0.7%); B.hominis + G.lamblia DNA - 1/308 (0.3%); D.fragilis + G.lamblia DNA
The analysis of data showed that 60/308 (19.5%) positive parasites DNA samples
were tested by microscopy: B.hominis – 30/141 (16/30 positive microscopy, 14/30
negative microscopy); D.fragilis – 20/91 (19/91 negative microscopy and 1/91
B.hominis positive microscopy but D.fragilis negative; Cryptosporidium spp. – 2/9
(negative microscopy); G.lamblia - 1/2 (negative microscopy); B.hominis + D.fragilis
– 6/57 (4/57 B.hominis positive microscopy, D.fragilis – negative; 2/57 negative
microscopy); B.hominis + Cryptosporidium spp. – 1/3 (B.hominis positive microscopy
but Cryptosporidium spp. negative).
Further investigation of parasites DNA samples showed that 583/891 (65.4%) were
negative by Multiplex PCR. 113/583 (19.4%) were tested by both methods: 108/583
(18.5%) were negative and 5/583 (0.9%) were positive by microscopy (4/5 -
B.hominis, 1/5 - E.histolytica) but negative by PCR.
Developments of Multiplex PCR-based assays have the potential to overcome
gastrointestinal tract parasite infections detection. From 308 positive parasite DNA
results mostly are found B.hominis and D.fragilis. 19.5% samples were tested by
PCR and microscopy: only 5.2% samples were positive by both methods, all
B.hominis, 1.6% - partially coincided and 12.6% were negative by microscopy,
however only 0,9% samples were negative by PCR but positive by microscopy.
|Period||22 Apr 2022 → 26 Apr 2022|
|Event title||32nd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)|
|Organiser||European Society of Clinical Microbiology and Infectious Diseases|
|Location||Lisbon, PortugalShow on map|
|Degree of Recognition||International|
- polymerase chain reaction
Documents & Links
- Abstract 04343
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