Platelet-Rich Fibrin Antibacterial Activity Against Klebsiella Pneumoniae

Activity: Talk or presentation types › Oral presentation

Description

Wound healing in the oral and maxillofacial region is compromised by infection. The antimicrobial properties of PRF play an important role in its successful application for tissue healing and regeneration. Last decade studies on PRF antimicrobial properties have increased and gained more attention and antimicrobial activity testing prevails using oral microbiomes.. Antibacterial activity testing was done using the agar-well diffusion method. Bacterial suspension of K. pneumoniae was inoculated on Mueller hinton agar (MHA) (Oxoid, UK). Peripheral blood samples from 17 healthy volunteers were drawn with the butterfly blood collection method in 10 mL S-PRF tubes. Tubes were placed in a centrifuge and centrifuged at 700 rpm for 3 min according to Choukroun's protocol to obtain i-PRF.  i-PRF was pipetted in prepared MHA wells and incubated at 37°C for 24 hours. Measures of zones of inhibition (ZOI) were done.. Experiments using K. pneumoniae reference cultures revealed moderate i-PRF antibacterial activity, showing ZOI (more than 5 mm) around MHA wells using samples from 10 patients.. Because of PRF’s inhomogeneous nature and clotting ability there is great importance in the use of the appropriate methodology to determine PRF’s antimicrobial effect and to get reliable results. i-PRF demonstrated antimicrobial activity against oral flora typical pathogens, but also to K. pneumoniae showing the perspective to widen PRF application. This research was funded by the European Union’s Horizon 2020 research and innovation program under the grant agreement No 857287 and the Latvian Council of Science research project No. lzp-2020/1-0054 (MATRI-X)”.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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