Platelet-Rich Fibrin Immunological Testing Using Elisa Assay

Activity: Talk or presentation types › Oral presentation

Description

The immunological properties of PRF rely on its ability to release autologous growth factors and cytokines in supraphysiologic concentrations. Various studies are describing ELISA immunological assay as the method to evaluate the concentration of various proteins in PRF. The lack of information in the literature regarding methodology and used PRF dilutions make protein evaluation a challenging task.. Peripheral blood samples from 18 volunteers were drawn with the butterfly blood collection method in 10 mL S-PRF tubes. Tubes were placed in a centrifuge and centrifuged at 700 rpm for 3 or 4 min according to Choukroun's protocol to obtain i-PRF and frozen immediately at -800C. Aliquots of i-PRF samples were prepared by thawing frozen i-PRF samples on ice and were frozen again. Before the performing ELISA assay, i-PRF samples aliquots were thawed on ice and placed at room temperature to clot for 30 minutes. The supernatant around clots was used to perform an ELISA assay according to instructions from kit manufacturer and i-PRF samples were diluted to make 4 different concentrations to be in the range of protein detection.. ELISA assay demonstrated high concentrations of TGFb1, EGF and VEGF in i-PRF. TGFb1 was found in the highest concentration compared to EGF and VEGF, which were more similar to each other. The differences in each patient’s protein content pattern were found. Tested proteins were in a range of detections using i-PRF supernatant dilutions: 1:4, 1:15, 1:100 and 1:200 for EGF; 1:1, 1:4, 1:20 for VEGF; and 1:100 and 1:400 for TGFb1.. i-PRF contains various growth factors in supraphysiological concentration. Between tested proteins TGFb1 is found to be in much higher concentrations. This research was funded by the European Union’s Horizon 2020 research and innovation program under the grant agreement No 857287 and the Latvian Council of Science research project No. lzp-2020/1-0054 (MATRI-X)”.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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