Potential Role of PAX7, PAX9, SHH, SOX3, WNT3A and WNT9B in Orofacial Cleft-Affected Palatal Tissue

Activity: Talk or presentation types › Oral presentation

Description

Non-syndromic cleft lip with or without a cleft palate is one of the most common congenital anomalies worldwide; however, morphopathogenesis of the clefts is not completely understood yet. Many candidate genes have been proposed to play a causal role, but the majority require further research. Taking into account the importance of PAX7, PAX9, SHH, SOX3, WNT3A and WNT9B in embryogenesis, the aim was to examine the appearance, distribution and potential intercorrelations between the six genes in cleft-affected palatine tissue.. The study group consisted of 15 children. The soft tissue samples were taken during veloplasty. Five control tissue samples from the same age group as the patients without any craniofacial pathologies were obtained from the archives at the Institute of Anatomy and Anthropology, Riga. The signals of the candidate genes were visualized using Chromogenic in-situ Hybridization. Nonparametric statistics, Mann-Whitney U and Spearman’s tests were used.. A statistically significant difference in the distribution of PAX7, PAX9, WNT3A and WNT9B in the epithelium between the study and control group was observed. The epithelium mostly expressed PAX7; however, PAX9, WNT3A, WNT9B and SOX3 were detected in a less distinct number of cells. The connective tissue cells showed only PAX7. In some cases, SHH was seen in an occasional number of cells. Nineteen statistically important pairs of positive correlations ranging from moderate to very strong were found between the genes.. Changes in the cleft-affected palatal epithelium mainly seem to be regulated by the PAX7 gene with some participation of PAX9, WNT3A, WNT9B and SOX3, while connective tissue changes depend on PAX7 only, but SHH seems to participate individually and indistinctly. Numerous positive correlations represent the gene interactions of the pathways in palatal cleft development.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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