Prevalence of Dental Microflora on the Adenoid Surface in Children with Adenoid Hyperplasia

Activity: Talk or presentation types › Poster presentation

Description

To identify dental and gingival bacterial microflora on genus level on the surface of adenoid tissue extracted from children undergoing adenoid operations in order to detect the level of dissimination of odontogenic microflora among other bacterial microorganisms.. Microbiological swabs of adenoid surface tissue extracted during elective adenotomy surgical procedure on pediatric patients (three to seven six old) who suffer from adenoid enlargement associated diseases. Microbial DNA was extracted using FastDNA, the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified. Data was imported into QIIME2, where we performed trimming of primer sequences to select reads matching the V3 and V4 hypervariable regions. The amplicon sequencing data are available in the European Nucleotide Archive.. Twenty-one sample was analyzed (11 male and 10 female, 2 to 6 years old) . Taxonomic analysis on phylum and genus level was conducted. Genus level analysis identified dental and buccal bacteria Veillonella atypica and Fusobacterium nucleatum on the adenoid surface in relative abundance, Veillonella genetic material attributing to 13% and Fusobacterium to 18% of total genetic material identified. Genetic material of Shaalia odontolytica, another common dental pathogen, was also represented in our samples, attributing to 7% of total genetic material. Phylum level analysis identified Firmicutes as the dominant phylum of bacterial microorganisms, followed by bacteroides.. Dental bacterial microflora is abundantly represented on adenoid surfaces of children suffering from adenoid hyperplasia. Genetic material of dental and gingival microflora (Veillonella atypica and Fusobacterium nucleatum ) attributed to 38% of total bacterial genetic material in our samples. Our findings suggest a more prominent migration of odontogenic microflora deeper into the nasopharynx and the adenoid surface.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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