Description
The aim of the study is to determine the allelic methylation status of the PSA proximal promoter in a prostate cancer (PCa) cell line model using PC3 and LNCaP, prostate cancer cell lines, and BPH-1 as a non-cancer control.. All cell lines were cultured at 37◦ C in a 5% CO2 atmosphere incubator according to company-provided protocol. The bisulfite treated DNA was used to amplify the promoter region of the PSA with primers specific for bisulfite converted DNA. 16-20 clones for each PCR product were sequenced and analysed. DNA sequencing was performed using the ABI BigDyeTerminator Cycle Sequencing Kit v3 and the sequences were detected on an ABI 3130XL Genetic Analyzer.. The methylation of 6 CpGs dinucleotides and 5 CCWGGs pentanucleotide motifs (W=A/T) harboured in PSA proximal promoter was detected in a prostate cancer cell line model. The allelic methylation status of the PSA promoter has shown that prostate cancer cell lines have characteristic allele-specific methylation ranging from: biallelically methylation-free – LNCaP (indolent PCa), having biallelic PSA expression; monoallelic CpG methylation accompanied by methylation of two proximal CCWGG sites of the same allele – BPH1 (non-cancer control); biallelic CpG promoter methylation, – PC3, PSA is not expressed. Lack of PSA expression, as in PC3 cells (aggressive PCa), due to biallelic methylation of the PSA promoter, in clinic will be lead to false negative results in PSA-based PCa testing due to gene silencing in a growing population of cancer cells. . Determining the allelic methylation status of the PSA promoter will explain the discrepancy between PSA levels and PCa disease in PSA-based PCa testing and can significantly improve PSA-based PCa analysis by translating promoter methylation status into specific PCa disease severity, according to the PCa cell line model study.| Period | 29 Mar 2023 → 31 Mar 2023 |
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| Event title | RSU International Research Conference 2023: Knowledge for Use in Practice |
| Event type | Conference |
| Organiser | Rīga Stradiņš University |
| Location | Riga, LatviaShow on map |
| Degree of Recognition | International |