Urinary Exosomal miRNA Profiles as Markers of Bladder Cancer Development

Activity: Talk or presentation types › Oral presentation

Description

The aim of this study is to find and to develop noninvasive molecular marker of the bladder cancer (BC) using urinary exosomal miRNAs. To understand the bladder cancer-related exosomal miRNAs we compared BC patients’ urinary exosomal miRNAs to healthy control.. The study analyzed urine samples from 11 BC (6 pTa or pT1 low-grade and 6 pT2 high-grade) and 7 healthy control patients, which were collected at Pauls Stradiņš Clinical University Hospital between 2018 and 2020. The urinary exosomal miRNAs in allgroups were analyzed on the RT-PCR based QIAGEN human miRNA panels (752 miRNAs). Subsequently, target genes of urinary exosomal miRNAs from low (LG) and high grade (HG) BC patients and their network analysis were predicted using miRTarget, STING, CytoNCA, and Cytoscape.. Among the 752 miRNAs analyzed, we found 96 and 78 differentially present urinary exosomal miRNAs altered with at least a 2.0-fold change in LG and HG group, respectively. 1425 genes were predicted as target genes of those miRNAs in LG group with 91 up-regulated miRNAs and 805 target genes with 71 up-regulated miRNAs in the HG group. Using these target genes, gene interaction networks with 20 key hub genes in each group were created. The interaction network predicted that TP53 is the strongest hub gene in both the LG and HG exosomal miRNA networks.. Identified exosomal miRNAs profiles cover many miRNAs which have been reported in bladder cancer tissues, plasma exosomes from BC patients, and BC patients urine. The miRNA profiles in this study are potentially used for the non-invasive molecular markers of BC.
Period29 Mar 2023
Event titleRSU International Research Conference 2023: Knowledge for Use in Practice
Event typeConference
OrganiserRīga Stradiņš University
LocationRiga, LatviaShow on map
Degree of RecognitionInternational

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