Prolonged hypoxia induced differential protein signatures in BRCA1 +/- triple negative breast cancer cell lines

Proposed project aims to analyse differential protein biosynthesis in BRCA1 +/- TNBC cell lines, cultured under prolonged hypoxia (to mimic in vivo microenvironment of breast cancer). Identified differentially expressed proteins will be analysed in respect to DSB repair signalling pathways to identify potential therapy targets for this aggressive type of breast cancer.
Project activities:
Protein isolation from cultured cell lines
Protein concentration determination and quality assessment with 1D gel electrophoresis
Sample preparation for mass-spectrometry
Sample analysis with LC-MSe/HDMSe
Data analysis with PLGS and bioinformatics
Identified proteins pathway analysis
Manuscript / conference thesis preparation for publication of acquired results