A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors

Ying Yang, Hajnalka Kiss, Maria Kost-Alimova, Darek Kedra, Ingegerd Fransson, Eyal Seroussi, Jingfeng Li, Anna Szeles, Irina Kholodnyuk, Marta P. Imreh, Katalin Fodor, Gyula Hadlaczky, George Klein, Jan P. Dumanski, Stephan Imreh

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalGenomics
Volume62
Issue number2
DOIs
Publication statusPublished - 1 Dec 1999
Externally publishedYes

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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