TY - JOUR
T1 - A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors
AU - Yang, Ying
AU - Kiss, Hajnalka
AU - Kost-Alimova, Maria
AU - Kedra, Darek
AU - Fransson, Ingegerd
AU - Seroussi, Eyal
AU - Li, Jingfeng
AU - Szeles, Anna
AU - Kholodnyuk, Irina
AU - Imreh, Marta P.
AU - Fodor, Katalin
AU - Hadlaczky, Gyula
AU - Klein, George
AU - Dumanski, Jan P.
AU - Imreh, Stephan
N1 - Funding Information:
We thank Kevin O’Brien for critically reviewing the manuscript. This work is supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Cancer Research Institute/Concern Foundation (New York, Los Angeles), the Cancer Society in Stockholm, the Berth von Kantzow Fond, and the Åke Wiberg’s Foundation.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999/12/1
Y1 - 1999/12/1
N2 - We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.
AB - We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.
UR - http://www.scopus.com/inward/record.url?scp=0033400204&partnerID=8YFLogxK
U2 - 10.1006/geno.1999.5952
DO - 10.1006/geno.1999.5952
M3 - Article
C2 - 10610706
AN - SCOPUS:0033400204
SN - 0888-7543
VL - 62
SP - 147
EP - 155
JO - Genomics
JF - Genomics
IS - 2
ER -