A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors

Ying Yang; Hajnalka Kiss; Maria Kost-Alimova; Darek Kedra; Ingegerd Fransson; Eyal Seroussi; Jingfeng Li; Anna Szeles; Irina Kholodnyuk; Marta P. Imreh; Katalin Fodor; Gyula Hadlaczky; George Klein; Jan P. Dumanski; Stephan Imreh

doi:10.1006/geno.1999.5952

A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors

Ying Yang
, Hajnalka Kiss
, Maria Kost-Alimova
, Darek Kedra
, Ingegerd Fransson
, Eyal Seroussi
, Jingfeng Li
, Anna Szeles
, Irina Kholodnyuk
, Marta P. Imreh
, Katalin Fodor
, Gyula Hadlaczky
, George Klein
, Jan P. Dumanski
, Stephan Imreh

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.

Original language	English
Pages (from-to)	147-155
Number of pages	9
Journal	Genomics
Volume	62
Issue number	2
DOIs	https://doi.org/10.1006/geno.1999.5952
Publication status	Published - 1 Dec 1999
Externally published	Yes

Field of Science*

1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1006/geno.1999.5952

Cite this

Yang, Y., Kiss, H., Kost-Alimova, M., Kedra, D., Fransson, I., Seroussi, E., Li, J., Szeles, A., Kholodnyuk, I., Imreh, M. P., Fodor, K., Hadlaczky, G., Klein, G., Dumanski, J. P., & Imreh, S. (1999). A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors. Genomics, 62(2), 147-155. https://doi.org/10.1006/geno.1999.5952

@article{f76a634fb98f4d94816687746a5601ed,

title = "A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors",

abstract = "We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.",

author = "Ying Yang and Hajnalka Kiss and Maria Kost-Alimova and Darek Kedra and Ingegerd Fransson and Eyal Seroussi and Jingfeng Li and Anna Szeles and Irina Kholodnyuk and Imreh, \{Marta P.\} and Katalin Fodor and Gyula Hadlaczky and George Klein and Dumanski, \{Jan P.\} and Stephan Imreh",

note = "Funding Information: We thank Kevin O{\textquoteright}Brien for critically reviewing the manuscript. This work is supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Cancer Research Institute/Concern Foundation (New York, Los Angeles), the Cancer Society in Stockholm, the Berth von Kantzow Fond, and the {\AA}ke Wiberg{\textquoteright}s Foundation. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "1999",

month = dec,

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doi = "10.1006/geno.1999.5952",

language = "English",

volume = "62",

pages = "147--155",

journal = "Genomics",

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T1 - A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors

AU - Yang, Ying

AU - Kiss, Hajnalka

AU - Kost-Alimova, Maria

AU - Kedra, Darek

AU - Fransson, Ingegerd

AU - Seroussi, Eyal

AU - Li, Jingfeng

AU - Szeles, Anna

AU - Kholodnyuk, Irina

AU - Imreh, Marta P.

AU - Fodor, Katalin

AU - Hadlaczky, Gyula

AU - Klein, George

AU - Dumanski, Jan P.

AU - Imreh, Stephan

N1 - Funding Information: We thank Kevin O’Brien for critically reviewing the manuscript. This work is supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Cancer Research Institute/Concern Foundation (New York, Los Angeles), the Cancer Society in Stockholm, the Berth von Kantzow Fond, and the Åke Wiberg’s Foundation. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1999/12/1

Y1 - 1999/12/1

N2 - We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.

AB - We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21.3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.

UR - https://www.scopus.com/pages/publications/0033400204

U2 - 10.1006/geno.1999.5952

DO - 10.1006/geno.1999.5952

M3 - Article

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AN - SCOPUS:0033400204

SN - 0888-7543

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ER -

A 1-MB PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors

Abstract

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