A 3p21.3 Region is preferentially eliminated from human chromosome 3/house microcell hybrids during tumor growth in SCID mice

Irina Kholodnyuk, Marija Kost-Alimova, Vladimir Kashuba, Rinat Gizatulin, Anna Szeles, Eric J. Stanbridge, Eugene R. Zabarovsky, George Klein, Stephan Imreh

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We have previously shown that four markers spanning the 3p24-p21.3 region, THRB, AP20R, D3S1029, and D3S32, were regularly eliminated from three human chromosome 3 (chr3)/mouse microcell hybrids (MCHs) during tumor growth in SCID mice. In an attempt to narrow down the eliminated region, we have studied 22 new SCID mouse tumors derived from 5 MCH lines carrying human chr3. They were analyzed by fluorescence in situ hybridization (FISH), Southern blotting, and polymerase chain reaction (PCR). MCHs that carried human chr1, chr8, chr13, and chr17 were examined as controls. We could identify a common eliminated region (CER) at 3p21.3, bordered distally by D3S1260 and proximally by D3S643/D3F15S2. Eight of 53 chr3-specific PCR markers, AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, AFMb362wb9, and D3S32, were localized within the CER. This finding is consistent with the notion that a tumor suppressor gene may be located in this area, as suggested by frequent loss of heterozygosity (LOH) within this region observed in several types of solid tumors.

Original languageEnglish
Pages (from-to)200-211
Number of pages12
JournalGenes Chromosomes and Cancer
Volume18
Issue number3
DOIs
Publication statusPublished - Mar 1997
Externally publishedYes

Field of Science

  • 1.6 Biological sciences
  • 3.2 Clinical medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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