A minibioreactor for developing "perfused" capillaries in cardiomyocyte aggregates

C. K. Chua, D. Liu, K. F. Leong, V. Mironov, V. Kasyanov

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Angiogenesis is very important in cancer formation and many other diseases as well as in tissue engineering. Its underlying regulation mechanism however remains largely unknown. Past in vitro studies on angiogenesis have achieved seeming capillary structure, but were unable to develop the key function of a normal blood vessel - the "perfusability". In the present study, a novel minibioreactor that helps develop "perfused" capillaries in cardiomyocyte aggregates is developed. This design allows cardiomyocyte aggregates loading and endothelial cell seeding in tandem. It attempts to promote capillary formation in cardiomyocyte aggregates, followed by capillary perfusion with the help of a dynamic perfusion loop. The minibioreactor allows easy onsite visual monitoring and can be located onto the platform of normal microscopes. This device will function as a convenient and easy-to-use device for studying the mechanisms of angiogenesis as well as the myocardiocyte behavior when cocultured with endothelial cells.

Original languageEnglish
Title of host publicationInnovative Developments in Design and Manufacturing
Subtitle of host publicationAdvanced Research in Virtual and Rapid Prototyping -- Proceedings of VRP4, Oct. 2009, Leiria, Portugal
Number of pages4
Publication statusPublished - 2010
Event4th International Conference on Advanced Research in Virtual and Physical Prototyping - Leiria, Portugal
Duration: 6 Oct 200910 Oct 2009
Conference number: 4


Conference4th International Conference on Advanced Research in Virtual and Physical Prototyping
Abbreviated titleVRAP 2009

Field of Science*

  • 2.6 Medical engineering
  • 3.4 Medical biotechnology

Publication Type*

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database


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