A monoclonal antibody affects chromophore apoprotein interactions of phytochrome A

Miki Nakazawa, Naoko Moriuchi, Naoko Wada, Tetsuya Hirose, Satoru Tokutomi, Katsushi Manabe

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A monoclonal antibody designated Mep-1 was raised against phytochrome A from pea (Pisum sativum L.). The binding of this antibody (class IgG1) to partially degraded phytochrome (114 kDa) caused a considerable increase in the far-red peak at the red-light-induced stationary state. The effect reached a plateau value when the antibody and phytochrome were present in approximately equimolar amounts. The dark transformation of the far-red-light-absorbing form to the red-light-absorbing form of the 114 kDa phytochrome was inhibited by the addition of the antibody. However, binding of the antibody to the undegraded 121 kDa phytochrome had no effects on the spectrum of the red-light-induced steady state. The site at which the antibody bound to phytochrome was determined to be between amino acid residues 256 and 383 of pea phytochrome A. This is the first report of a monoclonal antibody that enhances the farred absorption of phytochrome in the red-light-induced photostationary state.

Original languageEnglish
Pages (from-to)745-749
Number of pages5
JournalPhotochemistry and Photobiology
Volume65
Issue number4
DOIs
Publication statusPublished - Apr 1997
Externally publishedYes

Field of Science

  • 1.6 Biological sciences
  • 1.4 Chemical sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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