A novel hybrid multichannel biphasic calcium phosphate granule-based composite scaffold for cartilage tissue regeneration

Albert Jung, Preeti Makkar, Jhaleh Amirian , Byong Taek Lee (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


The objective of the present study was to develop a novel hybrid multichannel biphasic calcium phosphate granule (MCG)-based composite system for cartilage regeneration. First, hyaluronic acid-gelatin (HG) hydrogel was coated onto MCG matrix (MCG-HG). Poly(lactic-co-glycolic acid) (PLGA) microspheres was separately prepared and modified with polydopamine subsequent to BMP-7 loading (B). The surface-modified microspheres were finally embedded into MCG-HG scaffold to develop the novel hybrid (MCG-HG-PLGA-PD-B) composite system. The newly developed MCG-HG-PLGA-PD-B composite was then subjected to scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier Transform infrared spectroscopy, porosity, compressive strength, swelling, BMP-7 release and in-vitro biocompatibility studies. Results showed that 60% of BMP-7 retained on the granular surface after 28 days. A hybrid MCG-HG-PLGA-PD-B composite scaffold exhibited higher swelling and compressive strength compared to MCG-HG or MCG. In-vitro studies showed that MCG-HG-PLGA-PD-B had improved cell viability and cell proliferation for both MC3T3-E1 pre-osteoblasts and ATDC5 pre-chondrocytes cell line with respect to MCG-HG or MCG scaffold. Our results suggest that a hybrid MCG-HG-PLGA-PD-B composite scaffold can be a promising candidate for cartilage regeneration applications.
Original languageEnglish
Pages (from-to)775–787
Number of pages13
JournalJournal of Biomaterials Applications
Issue number6
Publication statusPublished - 13 Nov 2017
Externally publishedYes


  • Biphasic calcium phosphate granule
  • hyaluronic acid-gelatin
  • poly(lactic-co-glycolic acid) microspheres
  • bone morphogenetic protein 7
  • composite
  • cartilage regeneration

Field of Science*

  • 2.10 Nano-technology
  • 1.4 Chemical sciences
  • 2.5 Materials engineering
  • 2.6 Medical engineering

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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