A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling

Davaapurev Bekh-Ochir, Setsuko Shimada, Ayumi Yamagami, Satomi Kanda, Kenji Ogawa, Miki Nakazawa, Minami Matsui, Masaaki Sakuta, Hiroyuki Osada, Tadao Asami, Takeshi Nakano

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Plant steroid hormones, brassinosteroids, are essential for growth, development and responses to environmental stresses in plants. Although BR signaling proteins are localized in many organelles, i.e., the plasma membrane, nuclei, endoplasmic reticulum and vacuole, the details regarding the BR signaling pathway from perception at the cellular membrane receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) to nuclear events include several steps. Brz (Brz220) is a specific inhibitor of BR biosynthesis. In this study, we used Brz-mediated chemical genetics to identify Brz-insensitive-long hypocotyls 2-1D (bil2-1D). The BIL2 gene encodes a mitochondrial-localized DnaJ/Heat shock protein 40 (DnaJ/Hsp40) family, which is involved in protein folding. BIL2-overexpression plants (BIL2-OX) showed cell elongation under Brz treatment, increasing the growth of plant inflorescence and roots, the regulation of BR-responsive gene expression and suppression against the dwarfed BRI1-deficient mutant. BIL2-OX also showed resistance against the mitochondrial ATPase inhibitor oligomycin and higher levels of exogenous ATP compared with wild-type plants. BIL2 participates in resistance against salinity stress and strong light stress. Our results indicate that BIL2 induces cell elongation during BR signaling through the promotion of ATP synthesis in mitochondria.

Original languageEnglish
Pages (from-to)1509-1525
Number of pages17
JournalPlanta
Volume237
Issue number6
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Keywords

  • ATPase
  • Brassinosteroid
  • Cell elongation
  • Chemical biology
  • DnaJ/Hsp40
  • Mitochondria

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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