Age-related analysis of structural, biochemical and mechanical properties of the porcine mitral heart valve leaflets

Vladimir Kasyanov; Ricardo A. Moreno-Rodriguez; Martins Kalejs; Iveta Ozolanta; Peteris Stradins; Xuejun Wen; Hai Yao; Vladimir Mironov

doi:10.3109/03008207.2013.823954

Age-related analysis of structural, biochemical and mechanical properties of the porcine mitral heart valve leaflets

Vladimir Kasyanov, Ricardo A. Moreno-Rodriguez, Martins Kalejs, Iveta Ozolanta, Peteris Stradins, Xuejun Wen, Hai Yao, Vladimir Mironov

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The aim of this study was to assess structural and biochemical differences in the extracellular matrix of the fetal and adult porcine mitral heart valves in relation to their mechanical characteristics. Using tensile tests it was demonstrated that the material properties of porcine mitral heart valves progressively change with age. The collagen content of the adult heart valve, as estimated by hydroxyproline assay, increases three times as compared with fetal heart valves. Transmission electron microscopy demonstrated that the diameter of collagen fibrils increased in adult heart valves compared with fetal heart valves. The level of collagen cross-linking is lower in the fetal heart valve than the adult heart valve. The reported age differences in the material properties of fetal and adult porcine heart valves were associated with increases in collagen content, the diameter of collagen fibrils and the level of collagen cross-linking. These data lay a foundation for systematic elucidation of the structural determinants of material properties of heart valves during embryonic and postnatal valvulogenesis. They are also essential to define the desirable level of tissue maturation in heart valve tissue engineering.

Original language	English
Pages (from-to)	394-402
Number of pages	9
Journal	Connective Tissue Research
Volume	54
Issue number	6
DOIs	https://doi.org/10.3109/03008207.2013.823954
Publication status	Published - 2013

Keywords*

Biomechanics
Collagen
Heart valve
Porcine
Stress-strain

Field of Science*

2.5 Materials engineering
2.6 Medical engineering

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

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10.3109/03008207.2013.823954

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title = "Age-related analysis of structural, biochemical and mechanical properties of the porcine mitral heart valve leaflets",

abstract = "The aim of this study was to assess structural and biochemical differences in the extracellular matrix of the fetal and adult porcine mitral heart valves in relation to their mechanical characteristics. Using tensile tests it was demonstrated that the material properties of porcine mitral heart valves progressively change with age. The collagen content of the adult heart valve, as estimated by hydroxyproline assay, increases three times as compared with fetal heart valves. Transmission electron microscopy demonstrated that the diameter of collagen fibrils increased in adult heart valves compared with fetal heart valves. The level of collagen cross-linking is lower in the fetal heart valve than the adult heart valve. The reported age differences in the material properties of fetal and adult porcine heart valves were associated with increases in collagen content, the diameter of collagen fibrils and the level of collagen cross-linking. These data lay a foundation for systematic elucidation of the structural determinants of material properties of heart valves during embryonic and postnatal valvulogenesis. They are also essential to define the desirable level of tissue maturation in heart valve tissue engineering.",

keywords = "Biomechanics, Collagen, Heart valve, Porcine, Stress-strain",

author = "Vladimir Kasyanov and Moreno-Rodriguez, {Ricardo A.} and Martins Kalejs and Iveta Ozolanta and Peteris Stradins and Xuejun Wen and Hai Yao and Vladimir Mironov",

note = "Funding Information: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. This research is supported by NSF R-II grant {\textquoteleft}{\textquoteleft}South Carolina Project for Organ Biofabrication{\textquoteright}{\textquoteright}, Fondation Leducq Grant {\textquoteleft}{\textquoteleft}Mitral Valve Disease: From Genetic Mechanisms to Improved Repair{\textquoteright}{\textquoteright}; Latvian National Research Program in Medicine 2010–2013; {\textquoteleft}{\textquoteleft}Research of Biomaterials for Treatment of Cardiovascular Diseases{\textquoteright}{\textquoteright} project. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2013",

doi = "10.3109/03008207.2013.823954",

language = "English",

volume = "54",

pages = "394--402",

journal = "Connective Tissue Research",

issn = "0300-8207",

publisher = "Informa Healthcare",

number = "6",

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TY - JOUR

T1 - Age-related analysis of structural, biochemical and mechanical properties of the porcine mitral heart valve leaflets

AU - Kasyanov, Vladimir

AU - Moreno-Rodriguez, Ricardo A.

AU - Kalejs, Martins

AU - Ozolanta, Iveta

AU - Stradins, Peteris

AU - Wen, Xuejun

AU - Yao, Hai

AU - Mironov, Vladimir

N1 - Funding Information: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. This research is supported by NSF R-II grant ‘‘South Carolina Project for Organ Biofabrication’’, Fondation Leducq Grant ‘‘Mitral Valve Disease: From Genetic Mechanisms to Improved Repair’’; Latvian National Research Program in Medicine 2010–2013; ‘‘Research of Biomaterials for Treatment of Cardiovascular Diseases’’ project. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2013

Y1 - 2013

N2 - The aim of this study was to assess structural and biochemical differences in the extracellular matrix of the fetal and adult porcine mitral heart valves in relation to their mechanical characteristics. Using tensile tests it was demonstrated that the material properties of porcine mitral heart valves progressively change with age. The collagen content of the adult heart valve, as estimated by hydroxyproline assay, increases three times as compared with fetal heart valves. Transmission electron microscopy demonstrated that the diameter of collagen fibrils increased in adult heart valves compared with fetal heart valves. The level of collagen cross-linking is lower in the fetal heart valve than the adult heart valve. The reported age differences in the material properties of fetal and adult porcine heart valves were associated with increases in collagen content, the diameter of collagen fibrils and the level of collagen cross-linking. These data lay a foundation for systematic elucidation of the structural determinants of material properties of heart valves during embryonic and postnatal valvulogenesis. They are also essential to define the desirable level of tissue maturation in heart valve tissue engineering.

AB - The aim of this study was to assess structural and biochemical differences in the extracellular matrix of the fetal and adult porcine mitral heart valves in relation to their mechanical characteristics. Using tensile tests it was demonstrated that the material properties of porcine mitral heart valves progressively change with age. The collagen content of the adult heart valve, as estimated by hydroxyproline assay, increases three times as compared with fetal heart valves. Transmission electron microscopy demonstrated that the diameter of collagen fibrils increased in adult heart valves compared with fetal heart valves. The level of collagen cross-linking is lower in the fetal heart valve than the adult heart valve. The reported age differences in the material properties of fetal and adult porcine heart valves were associated with increases in collagen content, the diameter of collagen fibrils and the level of collagen cross-linking. These data lay a foundation for systematic elucidation of the structural determinants of material properties of heart valves during embryonic and postnatal valvulogenesis. They are also essential to define the desirable level of tissue maturation in heart valve tissue engineering.

KW - Biomechanics

KW - Collagen

KW - Heart valve

KW - Porcine

KW - Stress-strain

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M3 - Article

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ER -

Age-related analysis of structural, biochemical and mechanical properties of the porcine mitral heart valve leaflets

Abstract

Keywords*

Field of Science*

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