TY - JOUR
T1 - Antibacterial Activity of Tanacetum vulgare L. Extracts against Clinical Isolates of Bovine Mastitis
AU - Šukele, Renāte
AU - Bārzdiņa, Ance
AU - Koka, Rudīte
AU - Skadins, Ingus
AU - Lauberte, Līga
AU - Brangule, Agnese
AU - Kovalcuka, Liga
AU - Bandere, Dace
N1 - Funding Information:
This research was funded by the European Union’s Horizon 2020 research and innovation program under the grant agreement No 857287 and this research is supported by the Ministry of Agriculture and Rural Support Service Republic of Latvia with the European Agricultural Fund for Rural Development—Development of herbal plant containing medical extracts with anti-parasitic effect No 18-00-A01620-000028.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/3
Y1 - 2023/3
N2 - A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial activity against tested strains of S. aureus. The MIC and MBC values of a 70% ethanol extract of flowers (MIC = 3.4 mg/mL, MBC = 3.4–6.8 mg/mL) were lower than for the 70% ethanol extract of leaves (MIC = 15.7–31.4 mg/mL, MBC = 62.9–125.9 mg/mL). The flower extracts showed low activity against E. coli (MIC = 53.9 mg/mL, MBC = 53.9–107.8 mg/mL) and S. agalactiae (MIC, MBC = 53.9 mg/mL). T. vulgare leaf extracts had minimal antibacterial effects against Streptococcus strains (MIC = 31.4–62.9 mg/mL, MBC = 53.9–125.9 mg/mL) and Serratia liquefaciens (MIC, MBC = 125.9 mg/mL). However, flower extracts had a higher phenolic content that did not correlate with antibacterial effects. T. vulgare flower and leaf extracts could be combined to obtain broader antibacterial effects.
AB - A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial activity against tested strains of S. aureus. The MIC and MBC values of a 70% ethanol extract of flowers (MIC = 3.4 mg/mL, MBC = 3.4–6.8 mg/mL) were lower than for the 70% ethanol extract of leaves (MIC = 15.7–31.4 mg/mL, MBC = 62.9–125.9 mg/mL). The flower extracts showed low activity against E. coli (MIC = 53.9 mg/mL, MBC = 53.9–107.8 mg/mL) and S. agalactiae (MIC, MBC = 53.9 mg/mL). T. vulgare leaf extracts had minimal antibacterial effects against Streptococcus strains (MIC = 31.4–62.9 mg/mL, MBC = 53.9–125.9 mg/mL) and Serratia liquefaciens (MIC, MBC = 125.9 mg/mL). However, flower extracts had a higher phenolic content that did not correlate with antibacterial effects. T. vulgare flower and leaf extracts could be combined to obtain broader antibacterial effects.
KW - antibacterial activity
KW - bovine mastitis
KW - minimal bactericidal concentration
KW - minimum inhibitory concentration
KW - plant extracts
KW - Tanacetum vulgare
UR - http://www.scopus.com/inward/record.url?scp=85149988919&partnerID=8YFLogxK
U2 - 10.3390/app13053369
DO - 10.3390/app13053369
M3 - Article
AN - SCOPUS:85149988919
SN - 2076-3417
VL - 13
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
IS - 5
M1 - 3369
ER -
