Assessment of apoptosis and appearance of hepatocyte growth factor in placenta at different gestational ages: A cross-sectional study

Ilze Kreicberga, Anna Junga (Coresponding Author), Māra Pilmane

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Background: Fetal growth is determined by the interaction between mother and fetus using the placental interface throughout the pregnancy.
Objective: To research apoptosis and appearance of hepatocyte growth factor (HGF) in placentas of different gestational ages and to describe the anthropometrical and clinical indices of mothers and newborns.
Materials and Methods: The study material was obtained from 53 human immunodeficiency virus negative pregnant women of legal age without systemic
diseases. The staining of placental apoptotic cells was processed by a standard in
situ cell death detection kit. The detection of HGF was provided by the ImmunoCruz goat ABC Staining System protocol sc-2023. Relative distribution of positive structures was evaluated using the semiquantitative counting method.
Results: The mean rank value of the amount of HGF-containing cells (cytotrophoblasts, syncytiotrophoblasts, extravillous trophoblasts, Höfbauer cells, and cells of extraembryonic mesoderm) was 1.61 ± 0.94. Apoptotic cells (cytotrophoblasts, syncytiotrophoblasts, extravillous trophoblasts, and cells of extraembryonic mesoderm) were found in all placental samples of various gestational ages (term 13.00 ± 13.05 and preterm 27.00 ± 18.25); in general, their amount decreased with advancing gestational age of the placenta (p < 0.01).
Conclusion: Weight of a placenta directly depends on the gestational age and
correlates with the main fetal anthropometrical parameters (weight, length, and
head and chest circumferences). The decrease in HGF-containing and apoptotic
cells with advancing gestation depends on the adaptation potential of the placenta, proving the other ways of cellular disposition.
Original languageEnglish
Pages (from-to)505-514
Number of pages10
JournalInternational Journal of Reproductive BioMedicine
Issue number6
Publication statusPublished - Jun 2021


  • Pregnancy
  • Placenta
  • Gestational age
  • Apoptosis
  • Immunohistochemistry

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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