TY - JOUR
T1 - Assessment of exposure to pesticide mixtures in five European countries by a harmonized urinary suspect screening approach
AU - Ottenbros, Ilse
AU - Lebret, Erik
AU - Huber, Carolin
AU - Lommen, Arjen
AU - Antignac, Jean Philippe
AU - Čupr, Pavel
AU - Šulc, Libor
AU - Mikeš, Ondřej
AU - Szigeti, Tamás
AU - Középesy, Szilvia
AU - Martinsone, Inese
AU - Martinsone, Zanna
AU - Akulova, Lasma
AU - Pardo, Olga
AU - Fernández, Sandra F.
AU - Coscollá, Clara
AU - Pedraza-Diaz, Susana
AU - Krauss, Martin
AU - Debrauwer, Laurent
AU - Wagner, Kévin
AU - Nijssen, Rosalie
AU - Mol, Hans
AU - Vitale, Chiara Maria
AU - Klanova, Jana
AU - Molina, Borja Garlito
AU - León, Nuria
AU - Vermeulen, Roel
AU - Luijten, Mirjam
AU - Vlaanderen, Jelle
N1 - Funding Information:
This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 733032 HBM4EU, No 857560and No 857340. P.Č. O.M. & L.Š. acknowledge the RECETOX research infrastructure supported by the Ministry of Education, Youth and Sports of the Czech Republic (LM2018121) and the Ministry of Education, Youth and Sports of the Czech Republic (CZ.02.1.01/0.0/0.0/17_043/0009632). This publication reflects only the author's view and the European Commission is not responsible for any use that may be made of the information it contains. BG was supported by the Margarita Salas postdoctoral contract MGS/2021/25 (UP2021-021) financed by the European Union-NextGenerationEU.
Funding Information:
This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 733032 HBM4EU , No 857560 and No 857340 . P.Č., O.M. & L.Š. acknowledge the RECETOX research infrastructure supported by the Ministry of Education, Youth and Sports of the Czech Republic ( LM2018121 ) and the Ministry of Education, Youth and Sports of the Czech Republic ( CZ.02.1.01/0.0/0.0/17_043/0009632 ). This publication reflects only the author's view and the European Commission is not responsible for any use that may be made of the information it contains. BG was supported by the Margarita Salas postdoctoral contract MGS/2021/25 (UP2021-021) financed by the European Union-NextGenerationEU .
Publisher Copyright:
© 2022 The Authors
PY - 2023/3
Y1 - 2023/3
N2 - Humans are exposed to a mixture of pesticides through diet as well as through the environment. We conducted a suspect-screening based study to describe the probability of (concomitant) exposure to a set of pesticide profiles in five European countries (Latvia, Hungary, Czech Republic, Spain and the Netherlands). We explored whether living in an agricultural area (compared to living in a peri-urban area), being a a child (compared to being an adult), and the season in which the urine sample was collected had an impact on the probability of detection of pesticides (-metabolites). In total 2088 urine samples were collected from 1050 participants (525 parent-child pairs) and analyzed through harmonized suspect screening by five different laboratories. Fourty pesticide biomarkers (either pesticide metabolites or the parent pesticides as such) relating to 29 pesticides were identified at high levels of confidence in samples across all study sites. Most frequently detected were biomarkers related to the parent pesticides acetamiprid and chlorpropham. Other biomarkers with high detection rates in at least four countries related to the parent pesticides boscalid, fludioxonil, pirimiphos-methyl, pyrimethanil, clothianidin, fluazifop and propamocarb. In 84% of the samples at least two different pesticides were detected. The median number of detected pesticides in the urine samples was 3, and the maximum was 13 pesticides detected in a single sample. The most frequently co-occurring substances were acetamiprid with chlorpropham (in 62 urine samples), and acetamiprid with tebuconazole (30 samples). Some variation in the probability of detection of pesticides (-metabolites) was observed with living in an agricultural area or season of urine sampling, though no consistent patterns were observed. We did observe differences in the probability of detection of a pesticide (metabolite) among children compared to adults, suggesting a different exposure and/or elimination patterns between adults and children. This survey demonstrates the feasibility of conducting a harmonized pan-European sample collection, combined with suspect screening to provide insight in the presence of exposure to pesticide mixtures in the European population, including agricultural areas. Future improvements could come from improved (harmonized) quantification of pesticide levels.
AB - Humans are exposed to a mixture of pesticides through diet as well as through the environment. We conducted a suspect-screening based study to describe the probability of (concomitant) exposure to a set of pesticide profiles in five European countries (Latvia, Hungary, Czech Republic, Spain and the Netherlands). We explored whether living in an agricultural area (compared to living in a peri-urban area), being a a child (compared to being an adult), and the season in which the urine sample was collected had an impact on the probability of detection of pesticides (-metabolites). In total 2088 urine samples were collected from 1050 participants (525 parent-child pairs) and analyzed through harmonized suspect screening by five different laboratories. Fourty pesticide biomarkers (either pesticide metabolites or the parent pesticides as such) relating to 29 pesticides were identified at high levels of confidence in samples across all study sites. Most frequently detected were biomarkers related to the parent pesticides acetamiprid and chlorpropham. Other biomarkers with high detection rates in at least four countries related to the parent pesticides boscalid, fludioxonil, pirimiphos-methyl, pyrimethanil, clothianidin, fluazifop and propamocarb. In 84% of the samples at least two different pesticides were detected. The median number of detected pesticides in the urine samples was 3, and the maximum was 13 pesticides detected in a single sample. The most frequently co-occurring substances were acetamiprid with chlorpropham (in 62 urine samples), and acetamiprid with tebuconazole (30 samples). Some variation in the probability of detection of pesticides (-metabolites) was observed with living in an agricultural area or season of urine sampling, though no consistent patterns were observed. We did observe differences in the probability of detection of a pesticide (metabolite) among children compared to adults, suggesting a different exposure and/or elimination patterns between adults and children. This survey demonstrates the feasibility of conducting a harmonized pan-European sample collection, combined with suspect screening to provide insight in the presence of exposure to pesticide mixtures in the European population, including agricultural areas. Future improvements could come from improved (harmonized) quantification of pesticide levels.
KW - Co-occurrence
KW - Correlation patterns
KW - HBM4EU
KW - Mixtures
KW - Pesticide exposure
KW - Suspect screening
UR - http://www.scopus.com/inward/record.url?scp=85144776177&partnerID=8YFLogxK
U2 - 10.1016/j.ijheh.2022.114105
DO - 10.1016/j.ijheh.2022.114105
M3 - Review article
C2 - 36563507
AN - SCOPUS:85144776177
SN - 1438-4639
VL - 248
JO - International Journal of Hygiene and Environmental Health
JF - International Journal of Hygiene and Environmental Health
M1 - 114105
ER -