Abstract
Introduction: Persistent Helicobacter pylori (HP) infection may lead to development of chronic atrophic gastritis – important precancerous condition. Still it is not clear why mucosal damage occurs only in some patients infected with HP. Aim of the study was to find out if such HP virulence factors as cytotoxin associated gene A (CagA) and vacuolating cytotoxin A (VacA) are associated with the development of gastric mucosa atrophy.
Material and Methods: Patients (n = 304, median of age 61 years, males/females 113/191) due to current gastrointestinal symptoms underwent upper gastrointestinal tract endoscopy with standard biopsy sampling and further histopathological examination. Gastric mucosa atrophy was elevated according to Operative Link of Gastritis Assessment (OLGA). Patients having OLGA 0-I score were considered as non-atrophy patients and those with OLGA II-IV score – atrophy. Blood sample from each patient was collected; IgG for HP (Biohit, Finland) and anti-CagA and anti-VacA IgG (Mikrogen Diagnostik, Germany) were detected. Statistical analysis: chi-square test.
Results: HP infection rate among atrophy group was significantly higher compared to non-atrophy group (76.4% vs 63.8%, p = 0.025). Levels of anti-CagA and anti-VacA antibodies were significantly higher in atrophy group compared to non-atrophy group (correspondingly, 83.6% vs 62.4%, p < 0.001 and 22.7% vs 9.8%, p = 0.002).
Conclusions: Presence of anti-CagA and anti-VacA antibodies is associated with the development of gastric mucosa atrophy. Although other candidate HP virulence factors, predicting development of gastric mucosa damage, should be identified and their role have to be clarified.
Acknowledgements: Study was supported by ERDF project Nr.2010/0302/2DP/2.1.1.1.0/10/APIA/VIAA/158.
Material and Methods: Patients (n = 304, median of age 61 years, males/females 113/191) due to current gastrointestinal symptoms underwent upper gastrointestinal tract endoscopy with standard biopsy sampling and further histopathological examination. Gastric mucosa atrophy was elevated according to Operative Link of Gastritis Assessment (OLGA). Patients having OLGA 0-I score were considered as non-atrophy patients and those with OLGA II-IV score – atrophy. Blood sample from each patient was collected; IgG for HP (Biohit, Finland) and anti-CagA and anti-VacA IgG (Mikrogen Diagnostik, Germany) were detected. Statistical analysis: chi-square test.
Results: HP infection rate among atrophy group was significantly higher compared to non-atrophy group (76.4% vs 63.8%, p = 0.025). Levels of anti-CagA and anti-VacA antibodies were significantly higher in atrophy group compared to non-atrophy group (correspondingly, 83.6% vs 62.4%, p < 0.001 and 22.7% vs 9.8%, p = 0.002).
Conclusions: Presence of anti-CagA and anti-VacA antibodies is associated with the development of gastric mucosa atrophy. Although other candidate HP virulence factors, predicting development of gastric mucosa damage, should be identified and their role have to be clarified.
Acknowledgements: Study was supported by ERDF project Nr.2010/0302/2DP/2.1.1.1.0/10/APIA/VIAA/158.
| Original language | English |
|---|---|
| Article number | P03.14 |
| Pages (from-to) | 99 |
| Journal | Helicobacter |
| Volume | 18 |
| Issue number | Suppl.1 |
| Publication status | Published - Sept 2013 |
| Event | XXVIth International Workshop on Helicobacter and Related Bacteria in Chronic Digestive Inflammation and Gastric Cancer - Madrid, Spain Duration: 12 Sept 2013 → 14 Sept 2013 Conference number: 26 https://www.nutricion.ucr.ac.cr/index.php/es/proyectos/publicaciones/376-geographic-origin-of-helicobacter-pylori-isolated-from-costa-rican-patients-european-helicobacter-study-group-xxvith-international-workshop-on-helicobacter-and-related-bacteria-in-chronic-digestive-inflammation-and-gastric-cancer |
Field of Science*
- 3.2 Clinical medicine
- 3.1 Basic medicine
Publication Type*
- 3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database