Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients

Maurizio Cardelli; Remco Van Doorn; Lares Larcher; Michela Di Donato; Francesco Piacenza; Elisa Pierpaoli; Robertina Giacconi; Marco Malavolta; Sivaramakrishna Rachakonda; Nelleke A. Gruis; Anders Molven; Per Arne Andresen; Dace Pjanova; Joost J. Van Den Oord; Mauro Provinciali; Eduardo Nagore; Rajiv Kumar

doi:10.2217/epi-2020-0127

Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients

Maurizio Cardelli (Corresponding Author), Remco Van Doorn, Lares Larcher, Michela Di Donato, Francesco Piacenza, Elisa Pierpaoli, Robertina Giacconi, Marco Malavolta, Sivaramakrishna Rachakonda, Nelleke A. Gruis, Anders Molven, Per Arne Andresen, Dace Pjanova, Joost J. Van Den Oord, Mauro Provinciali, Eduardo Nagore, Rajiv Kumar

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Aim: To evaluate CpG methylation of long interspersed nuclear elements 1 (LINE-1) and human endogenous retrovirus K (HERV-K) retroelements as potential prognostic biomarkers in cutaneous melanoma. Materials methods: Methylation of HERV-K and LINE-1 retroelements was assessed in resected melanoma tissues from 82 patients ranging in age from 14 to 88 years. In addition, nevi from eight patients were included for comparison with nonmalignant melanocytic lesions. Results: Methylation levels were lower in melanomas than in nevi. HERV-K and LINE-1 methylation were decreased in melanoma patients with clinical parameters associated with adverse prognosis, while they were independent of age and gender. Hypomethylation of HERV-K (but not LINE-1) was an independent predictor of reduced disease-free survival. Conclusion: HERV-K hypomethylation can be a potential independent biomarker of melanoma recurrence.

Original language	English
Pages (from-to)	1689-1706
Number of pages	18
Journal	Epigenomics
Volume	12
Issue number	19
DOIs	https://doi.org/10.2217/epi-2020-0127
Publication status	Published - Oct 2020
Externally published	Yes

Keywords*

aging
disease-free survival
DNA methylation
HERV-K
HML-2
LINE-1
melanoma
prognosis
retrovirus
transposon

Field of Science*

1.6 Biological sciences
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/epi-2020-0127

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Cardelli, M., Doorn, R. V., Larcher, L., Donato, M. D., Piacenza, F., Pierpaoli, E., Giacconi, R., Malavolta, M., Rachakonda, S., Gruis, N. A., Molven, A., Andresen, P. A., Pjanova, D., Van Den Oord, J. J., Provinciali, M., Nagore, E., & Kumar, R. (2020). Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients. Epigenomics, 12(19), 1689-1706. https://doi.org/10.2217/epi-2020-0127

@article{917cd7a3b72746e2ae15cbcfbc347c5b,

title = "Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients",

abstract = "Aim: To evaluate CpG methylation of long interspersed nuclear elements 1 (LINE-1) and human endogenous retrovirus K (HERV-K) retroelements as potential prognostic biomarkers in cutaneous melanoma. Materials methods: Methylation of HERV-K and LINE-1 retroelements was assessed in resected melanoma tissues from 82 patients ranging in age from 14 to 88 years. In addition, nevi from eight patients were included for comparison with nonmalignant melanocytic lesions. Results: Methylation levels were lower in melanomas than in nevi. HERV-K and LINE-1 methylation were decreased in melanoma patients with clinical parameters associated with adverse prognosis, while they were independent of age and gender. Hypomethylation of HERV-K (but not LINE-1) was an independent predictor of reduced disease-free survival. Conclusion: HERV-K hypomethylation can be a potential independent biomarker of melanoma recurrence.",

keywords = "aging, disease-free survival, DNA methylation, HERV-K, HML-2, LINE-1, melanoma, prognosis, retrovirus, transposon",

author = "Maurizio Cardelli and Doorn, {Remco Van} and Lares Larcher and Donato, {Michela Di} and Francesco Piacenza and Elisa Pierpaoli and Robertina Giacconi and Marco Malavolta and Sivaramakrishna Rachakonda and Gruis, {Nelleke A.} and Anders Molven and Andresen, {Per Arne} and Dace Pjanova and {Van Den Oord}, {Joost J.} and Mauro Provinciali and Eduardo Nagore and Rajiv Kumar",

note = "Funding Information: As part of the European TRANSCAN project (JTC2013) “GENMEL” (Identification of genetic markers involved in development of metastases and second cancers in melanoma), this work was supported by the following grant: Italian Ministry of Health, TRANSCAN (JTC2013) - RRC-2015-2360467. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2020 ",

year = "2020",

month = oct,

doi = "10.2217/epi-2020-0127",

language = "English",

volume = "12",

pages = "1689--1706",

journal = "Epigenomics",

issn = "1750-1911",

publisher = "Future Medicine Ltd.",

number = "19",

}

Cardelli, M, Doorn, RV, Larcher, L, Donato, MD, Piacenza, F, Pierpaoli, E, Giacconi, R, Malavolta, M, Rachakonda, S, Gruis, NA, Molven, A, Andresen, PA, Pjanova, D, Van Den Oord, JJ, Provinciali, M, Nagore, E & Kumar, R 2020, 'Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients', Epigenomics, vol. 12, no. 19, pp. 1689-1706. https://doi.org/10.2217/epi-2020-0127

TY - JOUR

T1 - Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients

AU - Cardelli, Maurizio

AU - Doorn, Remco Van

AU - Larcher, Lares

AU - Donato, Michela Di

AU - Piacenza, Francesco

AU - Pierpaoli, Elisa

AU - Giacconi, Robertina

AU - Malavolta, Marco

AU - Rachakonda, Sivaramakrishna

AU - Gruis, Nelleke A.

AU - Molven, Anders

AU - Andresen, Per Arne

AU - Pjanova, Dace

AU - Van Den Oord, Joost J.

AU - Provinciali, Mauro

AU - Nagore, Eduardo

AU - Kumar, Rajiv

N1 - Funding Information: As part of the European TRANSCAN project (JTC2013) “GENMEL” (Identification of genetic markers involved in development of metastases and second cancers in melanoma), this work was supported by the following grant: Italian Ministry of Health, TRANSCAN (JTC2013) - RRC-2015-2360467. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2020

PY - 2020/10

Y1 - 2020/10

N2 - Aim: To evaluate CpG methylation of long interspersed nuclear elements 1 (LINE-1) and human endogenous retrovirus K (HERV-K) retroelements as potential prognostic biomarkers in cutaneous melanoma. Materials methods: Methylation of HERV-K and LINE-1 retroelements was assessed in resected melanoma tissues from 82 patients ranging in age from 14 to 88 years. In addition, nevi from eight patients were included for comparison with nonmalignant melanocytic lesions. Results: Methylation levels were lower in melanomas than in nevi. HERV-K and LINE-1 methylation were decreased in melanoma patients with clinical parameters associated with adverse prognosis, while they were independent of age and gender. Hypomethylation of HERV-K (but not LINE-1) was an independent predictor of reduced disease-free survival. Conclusion: HERV-K hypomethylation can be a potential independent biomarker of melanoma recurrence.

AB - Aim: To evaluate CpG methylation of long interspersed nuclear elements 1 (LINE-1) and human endogenous retrovirus K (HERV-K) retroelements as potential prognostic biomarkers in cutaneous melanoma. Materials methods: Methylation of HERV-K and LINE-1 retroelements was assessed in resected melanoma tissues from 82 patients ranging in age from 14 to 88 years. In addition, nevi from eight patients were included for comparison with nonmalignant melanocytic lesions. Results: Methylation levels were lower in melanomas than in nevi. HERV-K and LINE-1 methylation were decreased in melanoma patients with clinical parameters associated with adverse prognosis, while they were independent of age and gender. Hypomethylation of HERV-K (but not LINE-1) was an independent predictor of reduced disease-free survival. Conclusion: HERV-K hypomethylation can be a potential independent biomarker of melanoma recurrence.

KW - aging

KW - disease-free survival

KW - DNA methylation

KW - HERV-K

KW - HML-2

KW - LINE-1

KW - melanoma

KW - prognosis

KW - retrovirus

KW - transposon

UR - http://www.scopus.com/inward/record.url?scp=85097814340&partnerID=8YFLogxK

U2 - 10.2217/epi-2020-0127

DO - 10.2217/epi-2020-0127

M3 - Article

C2 - 33125285

AN - SCOPUS:85097814340

SN - 1750-1911

VL - 12

SP - 1689

EP - 1706

JO - Epigenomics

JF - Epigenomics

IS - 19

ER -

Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients

Abstract

Keywords*

Field of Science*

Publication Type*

UN SDGs

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