Associations between TNF-α, IL-6 and IL-10 Promoter Polymorphisms and Mortality in Severe Sepsis

Oļegs Sabeļņikovs, Liene Ņikitina-Zaķe, Angelika Krūmiņa, Zane Jaunberga, Jānis Klovins, Ludmila Vīksna, Lars J. Bjertnaes, Lilija Kovalchuka, Indulis Vanags

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: To determine whether an association exists between TNF-α308 A/G,IL-6174G/C, and IL-10-1082 A/G promoter polymorphisms and the corresponding systemic cytokine concentrations and outcome in patients suffering from sepsis.
Place and Duration of Study: The study was performed in the Intensive Care Unit (ICU)
of Pauls Stradins Clinical University Hospital, Riga. Between 1 August 2006 and 31 July2008. Methodology: We enrolled 103 consecutive intensive care unit patients with sepsis into a prospective case control study. Blood samples were obtained for extraction of DNA amplifying regions of interest by means of polymerase chain reaction technique (PCR)using specific primers for TNF-α, IL-6andIL-10. Simultaneously, plasma cytokines and standard laboratory variables were determined during the first 24 h after the diagnosis. Presence of septic shock, sequential organ failure assessment score (SOFA),demographic data and clinical outcome was noticed P < 0.05 was considered as statistically significant.
Results: Non-survivors had significantly higher concentrations of TNF-α, IL-6 and IL-10.The carriage of the IL-6-174C allele and IL-10-1082G allele were associated with a higher risk of mortality in patients with severe sepsis. Presence of the TNF-α-308 A allele did not influence mortality differently from those lacking this allele.
Conclusion: The present study demonstrated an association of the IL-6-174 and the IL-10-1082 with increased mortality in patients suffering from severe sepsis. We found no direct association between the examined polymorphisms and the corresponding cytokine levels.
Original languageEnglish
Article numberJSRR.2012.002
Pages (from-to)17-28
JournalJournal of Scientific Research and Reports
Volume1
Issue number1
Publication statusPublished - 2012

Keywords*

  • Sepsis
  • TNF-α
  • IL-6
  • IL-10
  • polymorphisms, allele
  • PCR
  • SNP

Field of Science*

  • 3.2 Clinical medicine

Publication Type*

  • 1.4. Reviewed scientific article published in Latvia or abroad in a scientific journal with an editorial board (including university editions)

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