Basic Fibroblast Growth Factor (bFGF), Fibroblast Growth Factor Receptor 1 (FGFR1), Transforming Growth Factor Beta (TGF-β) and Chromogranin A (CgA) Appearance in Congenital Intra-abdominal Adhesions in Children under One Year of Age

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Of this work was to determine dispersion of TGF-b, fibrosis modulating factor (bFGF and
FGR1) and granule marker chromogranin A in case of intra-abdominal adhesion which could be
essential factors in disease pathogenesis. Thus than could be used as possible biomarker.
Study Design: Observational study.
Place of Study: Institute of Anatomy and Anthropology and Department of Children Surgery, Rīga
Stradiņš University.
Materials and Methods: The specimens used for research were obtained from 50 patients aged 1
to 292 days. They underwent abdominal surgery due to obstructive gut malrotation and several
additional pathologies. Tissues were processed for bFGF, FGFR1, TGF-β and CgA by means of
biotin-streptavidin immunohistochemistry.
Results: In adhesion tissue bFGF positive connective tissue cells varied from a few to an
abundant amount, but in 15 specimens no positive structure was observed. Few connective tissue
fibers and moderate to numerous fibroblasts and macrophages contained FGFR1. A moderate
number of TGF-β positive connective tissue fibers were observed. Some specimens also contained positive fibroblasts, macrophages and endotheliocytes. Few connective tissue cells contained CgA. A moderate correlation was observed between bFGF and FGFR1 (Spearman’s rank correlation coefficient = .500, P < .001) as well as between bFGF and CgA (Spearman’s rank correlation coefficient = .311, P = .03).
Conclusions: The connection between the less distinct bFGF and more prominent FGFR1 proves
the compensatory stimulation of receptors as a response on the lack of the same factor in case of
adhesion disease. Persisting appearance of TGF-β positive structures in congenital adhesions
indicates the continuing growth/regeneration potential of loose connective tissue. Positive CgA
structures indicate the involvement of the neuroendocrine system in case of adhesion disease.
Original languageEnglish
Article number19676
Pages (from-to)1-9
JournalBritish Journal of Medicine and Medical Research
Volume10
Issue number11
DOIs
Publication statusPublished - 9 Sep 2015

Keywords*

  • children
  • adhesions
  • growth factors
  • immunohistochemistry

Field of Science*

  • 3.1 Basic medicine
  • 3.2 Clinical medicine

Publication Type*

  • 1.4. Reviewed scientific article published in Latvia or abroad in a scientific journal with an editorial board (including university editions)

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