Abstract
Aim: Of this work was to determine dispersion of TGF-b, fibrosis modulating factor (bFGF and
FGR1) and granule marker chromogranin A in case of intra-abdominal adhesion which could be
essential factors in disease pathogenesis. Thus than could be used as possible biomarker.
Study Design: Observational study.
Place of Study: Institute of Anatomy and Anthropology and Department of Children Surgery, Rīga
Stradiņš University.
Materials and Methods: The specimens used for research were obtained from 50 patients aged 1
to 292 days. They underwent abdominal surgery due to obstructive gut malrotation and several
additional pathologies. Tissues were processed for bFGF, FGFR1, TGF-β and CgA by means of
biotin-streptavidin immunohistochemistry.
Results: In adhesion tissue bFGF positive connective tissue cells varied from a few to an
abundant amount, but in 15 specimens no positive structure was observed. Few connective tissue
fibers and moderate to numerous fibroblasts and macrophages contained FGFR1. A moderate
number of TGF-β positive connective tissue fibers were observed. Some specimens also contained positive fibroblasts, macrophages and endotheliocytes. Few connective tissue cells contained CgA. A moderate correlation was observed between bFGF and FGFR1 (Spearman’s rank correlation coefficient = .500, P < .001) as well as between bFGF and CgA (Spearman’s rank correlation coefficient = .311, P = .03).
Conclusions: The connection between the less distinct bFGF and more prominent FGFR1 proves
the compensatory stimulation of receptors as a response on the lack of the same factor in case of
adhesion disease. Persisting appearance of TGF-β positive structures in congenital adhesions
indicates the continuing growth/regeneration potential of loose connective tissue. Positive CgA
structures indicate the involvement of the neuroendocrine system in case of adhesion disease.
FGR1) and granule marker chromogranin A in case of intra-abdominal adhesion which could be
essential factors in disease pathogenesis. Thus than could be used as possible biomarker.
Study Design: Observational study.
Place of Study: Institute of Anatomy and Anthropology and Department of Children Surgery, Rīga
Stradiņš University.
Materials and Methods: The specimens used for research were obtained from 50 patients aged 1
to 292 days. They underwent abdominal surgery due to obstructive gut malrotation and several
additional pathologies. Tissues were processed for bFGF, FGFR1, TGF-β and CgA by means of
biotin-streptavidin immunohistochemistry.
Results: In adhesion tissue bFGF positive connective tissue cells varied from a few to an
abundant amount, but in 15 specimens no positive structure was observed. Few connective tissue
fibers and moderate to numerous fibroblasts and macrophages contained FGFR1. A moderate
number of TGF-β positive connective tissue fibers were observed. Some specimens also contained positive fibroblasts, macrophages and endotheliocytes. Few connective tissue cells contained CgA. A moderate correlation was observed between bFGF and FGFR1 (Spearman’s rank correlation coefficient = .500, P < .001) as well as between bFGF and CgA (Spearman’s rank correlation coefficient = .311, P = .03).
Conclusions: The connection between the less distinct bFGF and more prominent FGFR1 proves
the compensatory stimulation of receptors as a response on the lack of the same factor in case of
adhesion disease. Persisting appearance of TGF-β positive structures in congenital adhesions
indicates the continuing growth/regeneration potential of loose connective tissue. Positive CgA
structures indicate the involvement of the neuroendocrine system in case of adhesion disease.
| Original language | English |
|---|---|
| Article number | 19676 |
| Pages (from-to) | 1-9 |
| Journal | British Journal of Medicine and Medical Research |
| Volume | 10 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 9 Sept 2015 |
Keywords*
- children
- adhesions
- growth factors
- immunohistochemistry
Field of Science*
- 3.1 Basic medicine
- 3.2 Clinical medicine
Publication Type*
- 1.4. Reviewed scientific article published in Latvia or abroad in a scientific journal with an editorial board (including university editions)