Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

Vladislav A. Parfenov (Coresponding Author), Elizaveta V. Koudan, Alisa A. Krokhmal, Elena A. Annenkova, Stanislav V. Petrov, Frederico D.A.S. Pereira, Pavel A. Karalkin, Elizaveta K. Nezhurina, Anna A. Gryadunova, Elena A. Bulanova, Oleg A. Sapozhnikov, Sergey A. Tsysar, Kaizheng Liu, Egbert Oosterwijk, Henk van Beuningen, Peter van der Kraan, Sanne Granneman, Hans Engelkamp, Peter Christianen, Vladimir KasyanovYusef D. Khesuani, Vladimir A. Mironov (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


In traditional tissue engineering, synthetic or natural scaffolds are usually used as removable temporal support, which involves some biotechnology limitations. The concept of “scaffield” approach utilizing the physical fields instead of biomaterial scaffold has been proposed recently. In particular, a combination of intense magnetic and acoustic fields can enable rapid levitational bioassembly of complex-shaped 3D tissue constructs from tissue spheroids at low concentration of paramagnetic agent (gadolinium salt) in the medium. In the current study, the tissue spheroids from human bladder smooth muscle cells (myospheres) are used as building blocks for assembling the tubular 3D constructs. Levitational assembly is accomplished at low concentrations of gadolinium salts in the high magnetic field at 9.5 T. The biofabricated smooth muscle constructs demonstrate contraction after the addition of vasoconstrictive agent endothelin-1. Thus, hybrid magnetoacoustic levitational bioassembly is considered as a new technology platform in the emerging field of formative biofabrication. This novel technology of scaffold-free, nozzle-free, and label-free bioassembly opens a unique opportunity for rapid biofabrication of 3D tissue and organ constructs with complex geometry.

Original languageEnglish
Article number2000721
JournalAdvanced healthcare materials
Issue number24
Publication statusPublished - 16 Dec 2020


  • levitation assembly
  • magnetoacoustic biofabrication
  • myospheres
  • tissue spheroids
  • urethral grafts

Field of Science*

  • 2.5 Materials engineering
  • 2.6 Medical engineering

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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