Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus

Youichi Kondou; Norifumi Mogami; Fumiko Hoshi; Shinsuke Kutsuna; Miki Nakazawa; Tetsuya Sakurai; Minami Matsui; Takakazu Kaneko; Satoshi Tabata; Ichiro Tanaka; Katsushi Manabe

doi:10.1093/pcp/pcf176

Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus

Youichi Kondou, Norifumi Mogami, Fumiko Hoshi, Shinsuke Kutsuna, Miki Nakazawa, Tetsuya Sakurai, Minami Matsui, Takakazu Kaneko, Satoshi Tabata, Ichiro Tanaka, Katsushi Manabe

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Abstract

We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.

Original language	English
Pages (from-to)	1585-1588
Number of pages	4
Journal	Plant and Cell Physiology
Volume	43
Issue number	12
DOIs	https://doi.org/10.1093/pcp/pcf176
Publication status	Published - 1 Dec 2002
Externally published	Yes

Keywords*

Bipolar localization
Methyl-accepting chemotaxis protein
Photoreceptor
Phototaxis
Phytochrome
Thermophilic cyanobacterium

Field of Science*

1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1093/pcp/pcf176

Bipolar Localization of Putative Photoreceptor ProteinFinal published version, 589 KB

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@article{a8d4980d9af84660b7a64d815efb855f,

title = "Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus",

abstract = "We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.",

keywords = "Bipolar localization, Methyl-accepting chemotaxis protein, Photoreceptor, Phototaxis, Phytochrome, Thermophilic cyanobacterium",

author = "Youichi Kondou and Norifumi Mogami and Fumiko Hoshi and Shinsuke Kutsuna and Miki Nakazawa and Tetsuya Sakurai and Minami Matsui and Takakazu Kaneko and Satoshi Tabata and Ichiro Tanaka and Katsushi Manabe",

note = "Funding Information: This work was supported in part by Grants-in-Aid for scientific research from the Ministry of Education, Science, Sports and Culture, Japan (no. 11640653 to K.M.).",

year = "2002",

month = dec,

day = "1",

doi = "10.1093/pcp/pcf176",

language = "English",

volume = "43",

pages = "1585--1588",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

number = "12",

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TY - JOUR

T1 - Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus

AU - Kondou, Youichi

AU - Mogami, Norifumi

AU - Hoshi, Fumiko

AU - Kutsuna, Shinsuke

AU - Nakazawa, Miki

AU - Sakurai, Tetsuya

AU - Matsui, Minami

AU - Kaneko, Takakazu

AU - Tabata, Satoshi

AU - Tanaka, Ichiro

AU - Manabe, Katsushi

N1 - Funding Information: This work was supported in part by Grants-in-Aid for scientific research from the Ministry of Education, Science, Sports and Culture, Japan (no. 11640653 to K.M.).

PY - 2002/12/1

Y1 - 2002/12/1

N2 - We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.

AB - We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.

KW - Bipolar localization

KW - Methyl-accepting chemotaxis protein

KW - Photoreceptor

KW - Phototaxis

KW - Phytochrome

KW - Thermophilic cyanobacterium

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U2 - 10.1093/pcp/pcf176

DO - 10.1093/pcp/pcf176

M3 - Article

C2 - 12514256

AN - SCOPUS:12244253793

SN - 0032-0781

VL - 43

SP - 1585

EP - 1588

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 12

ER -

Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus

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Keywords*

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