Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF

Jhaleh Amirian, Nguyen Thuy Ba Linh, Young Ki Min, Byong Taek Lee (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)


A composite scaffold of gelatin (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was fabricated for the successful delivery of growth factors. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were coated on the Gel-Pec-BCP surface to investigate of effect of them on bone healing. Surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive X-ray spectroscopy. The results obtained from Fourier transform infrared spectroscopy showed that BMP-2 and VEGF were successfully coated on Gel-Pec-BCP hydrogel scaffolds. MC3T3-E1 preosteoblasts were cultivated on the scaffolds to investigate the effect of BMP-2 and VEGF on cell viability and proliferation. VEGF and BMP-2 loaded on Gel-Pec-BCP scaffold facilitated increased cell spreading and proliferation compared to Gel-Pec-BCP scaffolds. In vivo, bone formation was examined using rat models. Bone formation was observed in Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds within 4 weeks, and was greatest with Gel-Pec-BCP/BMP-2 scaffolds. In vitro and in vivo results suggest that Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds could enhance bone regeneration.

Original languageEnglish
Pages (from-to)10-24
Number of pages15
JournalInternational Journal of Biological Macromolecules
Publication statusPublished - May 2015
Externally publishedYes


  • Biphase Calcium Phosphate (BCP)
  • BMP-2
  • Freeze-drying
  • Gelatin
  • Pectin
  • VEGF
  • in vivo studies
  • In vitro studies
  • composite materials

Field of Science*

  • 1.6 Biological sciences
  • 2.5 Materials engineering
  • 2.6 Medical engineering

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


Dive into the research topics of 'Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF'. Together they form a unique fingerprint.

Cite this