Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF

Jhaleh Amirian; Nguyen Thuy Ba Linh; Young Ki Min; Byong Taek Lee

doi:10.1016/j.ijbiomac.2015.02.021

Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF

Jhaleh Amirian, Nguyen Thuy Ba Linh, Young Ki Min, Byong Taek Lee (Corresponding Author)

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

A composite scaffold of gelatin (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was fabricated for the successful delivery of growth factors. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were coated on the Gel-Pec-BCP surface to investigate of effect of them on bone healing. Surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive X-ray spectroscopy. The results obtained from Fourier transform infrared spectroscopy showed that BMP-2 and VEGF were successfully coated on Gel-Pec-BCP hydrogel scaffolds. MC3T3-E1 preosteoblasts were cultivated on the scaffolds to investigate the effect of BMP-2 and VEGF on cell viability and proliferation. VEGF and BMP-2 loaded on Gel-Pec-BCP scaffold facilitated increased cell spreading and proliferation compared to Gel-Pec-BCP scaffolds. In vivo, bone formation was examined using rat models. Bone formation was observed in Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds within 4 weeks, and was greatest with Gel-Pec-BCP/BMP-2 scaffolds. In vitro and in vivo results suggest that Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds could enhance bone regeneration.

Original language	English
Pages (from-to)	10-24
Number of pages	15
Journal	International Journal of Biological Macromolecules
Volume	76
DOIs	https://doi.org/10.1016/j.ijbiomac.2015.02.021
Publication status	Published - May 2015
Externally published	Yes

Keywords*

Biphase Calcium Phosphate (BCP)
BMP-2
Freeze-drying
Gelatin
Pectin
VEGF
in vivo studies
In vitro studies
composite materials

Field of Science*

1.6 Biological sciences
2.5 Materials engineering
2.6 Medical engineering

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.ijbiomac.2015.02.021

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@article{5df6b96097d04f52b1125e3dd881fcd5,

title = "Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF",

abstract = "A composite scaffold of gelatin (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was fabricated for the successful delivery of growth factors. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were coated on the Gel-Pec-BCP surface to investigate of effect of them on bone healing. Surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive X-ray spectroscopy. The results obtained from Fourier transform infrared spectroscopy showed that BMP-2 and VEGF were successfully coated on Gel-Pec-BCP hydrogel scaffolds. MC3T3-E1 preosteoblasts were cultivated on the scaffolds to investigate the effect of BMP-2 and VEGF on cell viability and proliferation. VEGF and BMP-2 loaded on Gel-Pec-BCP scaffold facilitated increased cell spreading and proliferation compared to Gel-Pec-BCP scaffolds. In vivo, bone formation was examined using rat models. Bone formation was observed in Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds within 4 weeks, and was greatest with Gel-Pec-BCP/BMP-2 scaffolds. In vitro and in vivo results suggest that Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds could enhance bone regeneration.",

keywords = "Biphase Calcium Phosphate (BCP), BMP-2, Freeze-drying, Gelatin, Pectin, VEGF, in vivo studies, In vitro studies, composite materials",

author = "Jhaleh Amirian and Linh, {Nguyen Thuy Ba} and Min, {Young Ki} and Lee, {Byong Taek}",

note = "Funding Information: This work was supported by- Mid-carrer Researcher program through National Research Foundation of Korea (NRF) grant funded by Ministry of Education, Science and Technology (MEST) , and partially supported by Soonchunhyang university research fund. Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

month = may,

doi = "10.1016/j.ijbiomac.2015.02.021",

language = "English",

volume = "76",

pages = "10--24",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier",

}

TY - JOUR

T1 - Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF

AU - Amirian, Jhaleh

AU - Linh, Nguyen Thuy Ba

AU - Min, Young Ki

AU - Lee, Byong Taek

N1 - Funding Information: This work was supported by- Mid-carrer Researcher program through National Research Foundation of Korea (NRF) grant funded by Ministry of Education, Science and Technology (MEST) , and partially supported by Soonchunhyang university research fund. Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015/5

Y1 - 2015/5

N2 - A composite scaffold of gelatin (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was fabricated for the successful delivery of growth factors. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were coated on the Gel-Pec-BCP surface to investigate of effect of them on bone healing. Surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive X-ray spectroscopy. The results obtained from Fourier transform infrared spectroscopy showed that BMP-2 and VEGF were successfully coated on Gel-Pec-BCP hydrogel scaffolds. MC3T3-E1 preosteoblasts were cultivated on the scaffolds to investigate the effect of BMP-2 and VEGF on cell viability and proliferation. VEGF and BMP-2 loaded on Gel-Pec-BCP scaffold facilitated increased cell spreading and proliferation compared to Gel-Pec-BCP scaffolds. In vivo, bone formation was examined using rat models. Bone formation was observed in Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds within 4 weeks, and was greatest with Gel-Pec-BCP/BMP-2 scaffolds. In vitro and in vivo results suggest that Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds could enhance bone regeneration.

AB - A composite scaffold of gelatin (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was fabricated for the successful delivery of growth factors. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were coated on the Gel-Pec-BCP surface to investigate of effect of them on bone healing. Surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive X-ray spectroscopy. The results obtained from Fourier transform infrared spectroscopy showed that BMP-2 and VEGF were successfully coated on Gel-Pec-BCP hydrogel scaffolds. MC3T3-E1 preosteoblasts were cultivated on the scaffolds to investigate the effect of BMP-2 and VEGF on cell viability and proliferation. VEGF and BMP-2 loaded on Gel-Pec-BCP scaffold facilitated increased cell spreading and proliferation compared to Gel-Pec-BCP scaffolds. In vivo, bone formation was examined using rat models. Bone formation was observed in Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds within 4 weeks, and was greatest with Gel-Pec-BCP/BMP-2 scaffolds. In vitro and in vivo results suggest that Gel-Pec-BCP/BMP-2 and Gel-Pec-BCP/VEGF scaffolds could enhance bone regeneration.

KW - Biphase Calcium Phosphate (BCP)

KW - BMP-2

KW - Freeze-drying

KW - Gelatin

KW - Pectin

KW - VEGF

KW - in vivo studies

KW - In vitro studies

KW - composite materials

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U2 - 10.1016/j.ijbiomac.2015.02.021

DO - 10.1016/j.ijbiomac.2015.02.021

M3 - Article

C2 - 25709009

AN - SCOPUS:84924118982

SN - 0141-8130

VL - 76

SP - 10

EP - 24

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Bone formation of a porous Gelatin-Pectin-biphasic calcium phosphate composite in presence of BMP-2 and VEGF

Abstract

Keywords*

Field of Science*

Publication Type*

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