TY - GEN
T1 - Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats
AU - Krumina, G.
AU - Babarykin, D.
AU - Krumina, Z.
AU - Paegle, I.
AU - Suhorukov, O.
AU - Makarenkova, G.
AU - Nikulshin, S.
AU - Folkmane, I.
PY - 2013
Y1 - 2013
N2 - The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×106 cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.
AB - The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×106 cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.
KW - Bone marrow multipotent mesenchymal stromal cells
KW - polytrauma
UR - http://www.scopus.com/inward/record.url?scp=84870742236&partnerID=8YFLogxK
U2 - 10.1007/978-3-642-34197-7_53
DO - 10.1007/978-3-642-34197-7_53
M3 - Conference contribution
AN - SCOPUS:84870742236
SN - 9783642341960
VL - 38
T3 - IFMBE Proceedings
SP - 201
EP - 206
BT - International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012
T2 - International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012
Y2 - 10 October 2012 through 12 October 2012
ER -