Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats

G. Krumina; D. Babarykin; Z. Krumina; I. Paegle; O. Suhorukov; G. Makarenkova; S. Nikulshin; I. Folkmane

doi:10.1007/978-3-642-34197-7_53

Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats

G. Krumina (Corresponding Author), D. Babarykin, Z. Krumina, I. Paegle, O. Suhorukov, G. Makarenkova, S. Nikulshin, I. Folkmane

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×10⁶ cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.

Original language	English
Title of host publication	International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012
Pages	201-206
Number of pages	6
Volume	38
DOIs	https://doi.org/10.1007/978-3-642-34197-7_53
Publication status	Published - 2013
Externally published	Yes
Event	International Symposium on Biomedical Engineering and Medical Physics - Riga, Latvia Duration: 10 Oct 2012 → 12 Oct 2012 https://link.springer.com/book/10.1007/978-3-642-34197-7 http://www.bini.rtu.lv/isbemp/

Publication series

Name	IFMBE Proceedings
Volume	38 IFMBE
ISSN (Print)	1680-0737

Symposium

Symposium	International Symposium on Biomedical Engineering and Medical Physics
Abbreviated title	ISBEMP 2012
Country/Territory	Latvia
City	Riga
Period	10/10/12 → 12/10/12
Internet address	https://link.springer.com/book/10.1007/978-3-642-34197-7 http://www.bini.rtu.lv/isbemp/

Keywords*

Bone marrow multipotent mesenchymal stromal cells
polytrauma

Field of Science*

3.2 Clinical medicine

Publication Type*

3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database

Access to Document

10.1007/978-3-642-34197-7_53

Cite this

Krumina, G., Babarykin, D., Krumina, Z., Paegle, I., Suhorukov, O., Makarenkova, G., Nikulshin, S., & Folkmane, I. (2013). Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats. In International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012 (Vol. 38, pp. 201-206). (IFMBE Proceedings; Vol. 38 IFMBE). https://doi.org/10.1007/978-3-642-34197-7_53

@inproceedings{2c9120404b854c2d830a9059ec1ad12c,

title = "Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats",

abstract = "The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×106 cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.",

keywords = "Bone marrow multipotent mesenchymal stromal cells, polytrauma",

author = "G. Krumina and D. Babarykin and Z. Krumina and I. Paegle and O. Suhorukov and G. Makarenkova and S. Nikulshin and I. Folkmane",

year = "2013",

doi = "10.1007/978-3-642-34197-7_53",

language = "English",

isbn = "9783642341960",

volume = "38",

series = "IFMBE Proceedings",

pages = "201--206",

booktitle = "International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012",

note = "International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012 ; Conference date: 10-10-2012 Through 12-10-2012",

url = "https://link.springer.com/book/10.1007/978-3-642-34197-7, http://www.bini.rtu.lv/isbemp/",

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Krumina, G, Babarykin, D, Krumina, Z, Paegle, I, Suhorukov, O, Makarenkova, G, Nikulshin, S & Folkmane, I 2013, Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats. in International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012. vol. 38, IFMBE Proceedings, vol. 38 IFMBE, pp. 201-206, International Symposium on Biomedical Engineering and Medical Physics, Riga, Latvia, 10/10/12. https://doi.org/10.1007/978-3-642-34197-7_53

Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats. / Krumina, G. (Corresponding Author); Babarykin, D.; Krumina, Z. et al.
International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012. Vol. 38 2013. p. 201-206 (IFMBE Proceedings; Vol. 38 IFMBE).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats

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AU - Babarykin, D.

AU - Krumina, Z.

AU - Paegle, I.

AU - Suhorukov, O.

AU - Makarenkova, G.

AU - Nikulshin, S.

AU - Folkmane, I.

PY - 2013

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N2 - The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×106 cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.

AB - The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×106 cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.

KW - Bone marrow multipotent mesenchymal stromal cells

KW - polytrauma

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Bone marrow multipotent mesenchymal stromal cells transplantation effects after experimental polytrauma in rats

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Keywords*

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