Abstract
The objective of this study was to develop novel porous composite scaffolds for bone tissue engineering through surface modification of polycaprolactone–biphasic calcium phosphate-based composites (PCL–BCP). PCL–BCP composites were first fabricated with salt-leaching method followed by aminolysis. Layer by layer (LBL) technique was then used to immobilize collagen (Col) and bone morphogenetic protein (BMP-2) on PCL–BCP scaffolds to develop PCL–BCP–Col–BMP-2 composite scaffold. The morphology of the composite was examined by scanning electron microscopy (SEM). The efficiency of grafting of Col and BMP-2 on composite scaffold was measured by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Both XPS and FTIR confirmed that Col and BMP-2 were successfully immobilized into PCL–BCP composites. MC3TC3-E1 preosteoblasts cells were cultivated on composites to determine the effect of Col and BMP-2 immobilization on cell viability and proliferation. PCL–BCP–Col–BMP-2 showed more cell attachment, cell viability, and proliferation bone factors compared to PCL–BCP-Col composites. In addition, in vivo bone formation study using rat models showed that PCL–BCP–Col–BMP-2 composites had better bone formation than PCL–BCP-Col scaffold in critical size defect with 4 weeks of duration. These results suggest that PCL–BCP–Col–BMP-2 composites can enhance bone regeneration in critical size defect in a rat model with 4 weeks of duration.
Original language | English |
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Article number | 45186 |
Number of pages | 11 |
Journal | Journal of Applied Polymer Science |
Volume | 134 |
Issue number | 33 |
DOIs | |
Publication status | Published - 2 May 2017 |
Externally published | Yes |
Keywords*
- composite
- in vivo
- LBL technique
- PCL-BCP-Col-BMP-2
- Surface modification
Field of Science*
- 1.4 Chemical sciences
- 2.5 Materials engineering
- 3.4 Medical biotechnology
Publication Type*
- 1.1. Scientific article indexed in Web of Science and/or Scopus database