Bone morphogenetic protein-2 immobilization on porous PCL-BCP-Col composite scaffolds for bone tissue engineering

Myeong-Jin Song, Jhaleh Amirian , Nguyen Thuy Ba Linh, Byong-Taek Lee (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


The objective of this study was to develop novel porous composite scaffolds for bone tissue engineering through surface modification of polycaprolactone–biphasic calcium phosphate-based composites (PCL–BCP). PCL–BCP composites were first fabricated with salt-leaching method followed by aminolysis. Layer by layer (LBL) technique was then used to immobilize collagen (Col) and bone morphogenetic protein (BMP-2) on PCL–BCP scaffolds to develop PCL–BCP–Col–BMP-2 composite scaffold. The morphology of the composite was examined by scanning electron microscopy (SEM). The efficiency of grafting of Col and BMP-2 on composite scaffold was measured by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Both XPS and FTIR confirmed that Col and BMP-2 were successfully immobilized into PCL–BCP composites. MC3TC3-E1 preosteoblasts cells were cultivated on composites to determine the effect of Col and BMP-2 immobilization on cell viability and proliferation. PCL–BCP–Col–BMP-2 showed more cell attachment, cell viability, and proliferation bone factors compared to PCL–BCP-Col composites. In addition, in vivo bone formation study using rat models showed that PCL–BCP–Col–BMP-2 composites had better bone formation than PCL–BCP-Col scaffold in critical size defect with 4 weeks of duration. These results suggest that PCL–BCP–Col–BMP-2 composites can enhance bone regeneration in critical size defect in a rat model with 4 weeks of duration.
Original languageEnglish
Article number45186
Number of pages11
JournalJournal of Applied Polymer Science
Issue number33
Publication statusPublished - 2 May 2017
Externally publishedYes


  • composite
  • in vivo
  • LBL technique
  • PCL-BCP-Col-BMP-2
  • Surface modification

Field of Science*

  • 1.4 Chemical sciences
  • 2.5 Materials engineering
  • 3.4 Medical biotechnology

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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