TY - JOUR
T1 - BRZ-INSENSITIVE-PALE GREEN 1 is encoded by chlorophyll biosynthesis enzyme gene that functions in the downstream of brassinosteroid signaling
AU - Tachibana, Ryo
AU - Yamagami, Ayumi
AU - Miyagi, Shino
AU - Nakazawa (Miklasevica), Miki
AU - Matsui, Minami
AU - Sakuta, Masaaki
AU - Tanaka, Ryouichi
AU - Asami, Tadao
AU - Nakano, Takeshi
N1 - Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2022/8/1
Y1 - 2022/8/1
N2 - Brassinosteroids (BRs), a kind of phytohormone, have various biological activities such as promoting plant growth, increasing stress resistance, and chloroplast development. Though BRs have been known to have physiological effects on chloroplast, the detailed mechanism of chloroplast development and chlorophyll biosynthesis in BR signaling remains unknown. Here we identified a recessive pale green Arabidopsis mutant, Brz-insensitive-pale green1 (bpg1), which was insensitive to promoting of greening by BR biosynthesis-specific inhibitor Brz in the light. BPG1 gene encoded chlorophyll biosynthesis enzyme, 3, 8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), and bpg1 accumulated divinyl chlorophylls. Chloroplast development was suppressed in bpg1. Brz dramatically increased the expression of chlorophyll biosynthesis enzyme genes, including BPG1. These results suggest that chlorophyll biosynthesis enzymes are regulated by BR signaling in the aspect of gene expression and BPG1 plays an important role in regulating chloroplast development.
AB - Brassinosteroids (BRs), a kind of phytohormone, have various biological activities such as promoting plant growth, increasing stress resistance, and chloroplast development. Though BRs have been known to have physiological effects on chloroplast, the detailed mechanism of chloroplast development and chlorophyll biosynthesis in BR signaling remains unknown. Here we identified a recessive pale green Arabidopsis mutant, Brz-insensitive-pale green1 (bpg1), which was insensitive to promoting of greening by BR biosynthesis-specific inhibitor Brz in the light. BPG1 gene encoded chlorophyll biosynthesis enzyme, 3, 8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), and bpg1 accumulated divinyl chlorophylls. Chloroplast development was suppressed in bpg1. Brz dramatically increased the expression of chlorophyll biosynthesis enzyme genes, including BPG1. These results suggest that chlorophyll biosynthesis enzymes are regulated by BR signaling in the aspect of gene expression and BPG1 plays an important role in regulating chloroplast development.
KW - brassinosteroid
KW - chlorophyll biosynthesis
UR - http://www.scopus.com/inward/record.url?scp=85134855241&partnerID=8YFLogxK
U2 - 10.1093/bbb/zbac071
DO - 10.1093/bbb/zbac071
M3 - Article
SN - 0916-8451
VL - 86
SP - 1041
EP - 1048
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 8
ER -