Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement

Paulius Danilevicius; Rodrigo A. Rezende; Frederico D.A.S. Pereira; Alexandros Selimis; Vladimir Kasyanov; Pedro Y. Noritomi; Jorge V.L. da Silva; Maria Chatzinikolaidou; Maria Farsari; Vladimir Mironov

doi:10.1116/1.4922646

Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement

Paulius Danilevicius, Rodrigo A. Rezende, Frederico D.A.S. Pereira, Alexandros Selimis, Vladimir Kasyanov, Pedro Y. Noritomi, Jorge V.L. da Silva, Maria Chatzinikolaidou, Maria Farsari, Vladimir Mironov

Institute of Anatomy and Anthropology

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled using finite elements analysis and experimentally measured using a Microsquisher^®. They are subsequently loaded with preosteoblastic cells, which remain live after 24 and 72 h. The interlockable scaffolds have maintained their ability to fuse with tissue spheroids. This work represents a novel technological platform, enabling the rapid, laser-based, in situ 3D tissue biofabrication.

Original language	English
Article number	021011
Journal	Biointerphases
Volume	10
Issue number	2
DOIs	https://doi.org/10.1116/1.4922646
Publication status	Published - 23 Jun 2015

Field of Science*

2.5 Materials engineering

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

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10.1116/1.4922646

Cite this

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title = "Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement",

abstract = "The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled using finite elements analysis and experimentally measured using a Microsquisher{\textregistered}. They are subsequently loaded with preosteoblastic cells, which remain live after 24 and 72 h. The interlockable scaffolds have maintained their ability to fuse with tissue spheroids. This work represents a novel technological platform, enabling the rapid, laser-based, in situ 3D tissue biofabrication.",

author = "Paulius Danilevicius and Rezende, {Rodrigo A.} and Pereira, {Frederico D.A.S.} and Alexandros Selimis and Vladimir Kasyanov and Noritomi, {Pedro Y.} and {da Silva}, {Jorge V.L.} and Maria Chatzinikolaidou and Maria Farsari and Vladimir Mironov",

note = "Publisher Copyright: {\textcopyright} 2015 American Vacuum Society.",

year = "2015",

month = jun,

day = "23",

doi = "10.1116/1.4922646",

language = "English",

volume = "10",

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T1 - Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement

AU - Danilevicius, Paulius

AU - Rezende, Rodrigo A.

AU - Pereira, Frederico D.A.S.

AU - Selimis, Alexandros

AU - Kasyanov, Vladimir

AU - Noritomi, Pedro Y.

AU - da Silva, Jorge V.L.

AU - Chatzinikolaidou, Maria

AU - Farsari, Maria

AU - Mironov, Vladimir

PY - 2015/6/23

Y1 - 2015/6/23

N2 - The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled using finite elements analysis and experimentally measured using a Microsquisher®. They are subsequently loaded with preosteoblastic cells, which remain live after 24 and 72 h. The interlockable scaffolds have maintained their ability to fuse with tissue spheroids. This work represents a novel technological platform, enabling the rapid, laser-based, in situ 3D tissue biofabrication.

AB - The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled using finite elements analysis and experimentally measured using a Microsquisher®. They are subsequently loaded with preosteoblastic cells, which remain live after 24 and 72 h. The interlockable scaffolds have maintained their ability to fuse with tissue spheroids. This work represents a novel technological platform, enabling the rapid, laser-based, in situ 3D tissue biofabrication.

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ER -

Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement

Abstract

Field of Science*

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