Characterisation and in Vivo Safety of Canine Adipose-Derived Stem Cells

Uldis Berziņš, Ilze Matise-VanHoutana, Ilze Petersone, Ilmars Duritis, Sergejs Ņikuļšins, Ance Bogdanova-Jatniece, Martiņš Kalis, Šimons Svirskis, Dace Skrastiņa, Agnese Ezerta, Tatjana Kozlovska

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The study characterises canine adipose-derived stem cells (cASCs) in comparison to human ASCs (hASCs) and tests their safety in a canine model after intravenous administration. cASCs from two dogs were cultured under hypoxic conditions in a medium supplemented with autologous serum. They were plastic adherent, spindle-shaped cells that expressed CD73, CD90, and CD44 but lacked CD45, CD14, HLA-DR, and CD34. cASCs differentiated toward adipogenic, osteogenic, and chondrogenic lineages, although adipogenic differentiation capacity was low. Blast transformation reaction demonstrated that these cells significantly suppress T-cell proliferation, and this ability is dose-dependent. Intravenous administration of a cell freezing medium, therapeutic dose of cASCs (2 × 106 live cells/kg), and five times higher dose of cASCs showed no significant side effects in two dogs. Microscopic tissue lesions were limited to only mild, non-specific changes. There were no signs of malignancy. The results of the study indicate that cASCs are similar to hASCs and are safe for therapeutic applications in a canine model. The proposed methodology for ASC preparation on a non-routine basis, which includes individually optimised cell culture conditions and offers risk-adapted treatment, could be used for future personalised off-the-shelf therapies, for example, in myocardial infarction or stroke.

Original languageEnglish
Pages (from-to)160-171
Number of pages12
JournalProceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
Issue number3
Publication statusPublished - 1 Jun 2018


  • advanced therapy medicinal products
  • autologous adipose-derived stem cells
  • hospital exemption
  • pulmonary first-pass effect
  • stem cell safety

Field of Science*

  • 3.1 Basic medicine
  • 3.2 Clinical medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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