Characterization of a Pinus sylvestris thaumatin-like protein gene and determination of antimicrobial activity of the in vitro expressed protein

Ilze Šņepste, Vilnis Šķipars, Baiba Krivmane, Lauma Brūna, Dainis Ruņģis

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Thaumatin-like proteins (TLPs) are pathogenesis-related proteins, which are involved in plant defense responses to pathogen infection. Expression of the Pinus sylvestris L. TLP gene is up-regulated by methyl jasmonate treatment and inoculation with Heterobasidion annosum. A full-length Pinus taeda TLP gene sequence was used to design PCR primers for amplification of the full-length TLP gene from P. sylvestris. A putative 705-bp open reading frame of TLP gene was cloned into Escherichia coli cells, and then subcloned into the overexpression vector pET100 using BL21 Star expression bacteria. Optimization of the expression of recombinant TLP was achieved by decreasing both expression temperature and IPTG concentration. The purified 24.6-kDa TLP shows antimicrobial activity against 12 fungal species.

Original languageEnglish
Article number58
Number of pages13
JournalTree Genetics and Genomes
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Aug 2018
Externally publishedYes

Keywords*

  • Antimicrobial activity
  • Methyl jasmonate
  • Pinus sylvestris
  • Protein expression
  • Thaumatin-like protein

Field of Science*

  • 1.6 Biological sciences
  • 4.1 Agriculture, Forestry, and Fisheries

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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