Characterization of atherosclerotic arterial tissue using combined SHG and FLIM microscopy

Riccardo Cicchi (Coresponding Author), Enrico Baria, Christian Matthäus, Marta Lange, Annika Lattermann, Bernhard R. Brehm, Jürgen Popp, Francesco S. Pavone

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Atherosclerosis is among the most widespread cardiovascular diseases and one of the leading cause of death in the Western World. Characterization of arterial tissue in atherosclerotic condition is extremely interesting from the diagnostic point of view, especially for what is concerning collagen content and organization because collagen plays a crucial role in plaque vulnerability. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires immunehistochemical examination and a morpho-functional approach. Non-linear microscopy techniques offer the potential for providing morpho-functional information on the examined tissues in a label-free way. In this study, we employed combined SHG and FLIM microscopy for characterizing collagen organization in both normal arterial wall and within atherosclerotic plaques. Image pattern analysis of SHG images allowed characterizing collagen organization in different tissue regions. In addition, the analysis of collagen fluorescence decay contributed to the characterization of the samples based on collagen fluorescence lifetime. Different values of collagen fiber mean size, collagen distribution, and collagen anisotropy and collagen fluorescence lifetime were found in normal arterial wall and within plaque depositions, prospectively allowing for automated classification of atherosclerotic lesions and plaque vulnerability. The presented method represents a promising diagnostic tool for evaluating atherosclerotic tissue and has the potential to find a stable place in clinical setting as well as to be applied in vivo in the near future.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2015
PublisherOptica Publishing Group
Number of pages4
ISBN (Print)9781628417012
Publication statusPublished - Jul 2015
Externally publishedYes
EventEuropean Conference on Biomedical Optics (ECBO) - ICM-International Congress Center, Munich, Germany
Duration: 21 Jun 201525 Jun 2015

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701


ConferenceEuropean Conference on Biomedical Optics (ECBO)
Abbreviated titleECBO 2015
Internet address


  • Nonlinear microscopy
  • Scanning microscopy
  • Tissue characterization

Field of Science*

  • 1.3 Physical sciences
  • 2.6 Medical engineering
  • 3.2 Clinical medicine

Publication Type*

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database


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